Title |
MiR-278-3p regulates pyrethroid resistance in Culex pipiens pallens
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Published in |
Parasitology Research, November 2014
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DOI | 10.1007/s00436-014-4236-7 |
Pubmed ID | |
Authors |
Zhentao Lei, Yuan Lv, Weijie Wang, Qin Guo, Feifei Zou, Shengli Hu, Fujin Fang, Mengmeng Tian, Bingqian Liu, Xianmiao Liu, Kai Ma, Lei Ma, Dan Zhou, Donghui Zhang, Yan Sun, Bo Shen, Changliang Zhu |
Abstract |
MicroRNAs (miRNAs) regulate gene expression and biological processes including embryonic development, innate immunity, and infection in many species. Emerging evidence indicates that miRNAs are involved in drug resistance. However, little is known about the relationship between the miRNAs and insecticide resistance in mosquitos. Here, we reported that conserved miR-278-3p and its target gene are critical for pyrethroid resistance in Culex pipiens pallens. We found that CYP6AG11 is the target of miR-278-3p, through bioinformatic analysis and experimental verification. The expression level of miR-278-3p was lower, whereas the level of CYP6AG11 was higher in deltamethrin-resistant strain, which were detected using quantitative reverse transcription PCR (qRT-PCR). We also found that CYP6AG11 was regulated by miR-278-3p via a specific target site with the 3' untranslated region (UTR) by luciferase reporter assay. In addition, overexpression of CYP6AG11 in the mosquito C6/36 cells showed better proliferation than the cells with empty vector when treated by deltamethrin at different concentrations. Moreover, the overexpression of miR-278-3p through microinjection led to a significant reduction in the survival rate, and the level of CYP6AG11 was simultaneously reduced. These results indicated that miR-278-3p could regulate the pyrethroid resistance through CYP6AG11. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 3% |
Unknown | 28 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 5 | 17% |
Researcher | 4 | 14% |
Student > Ph. D. Student | 4 | 14% |
Other | 3 | 10% |
Student > Bachelor | 3 | 10% |
Other | 3 | 10% |
Unknown | 7 | 24% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 9 | 31% |
Biochemistry, Genetics and Molecular Biology | 4 | 14% |
Medicine and Dentistry | 4 | 14% |
Environmental Science | 1 | 3% |
Earth and Planetary Sciences | 1 | 3% |
Other | 3 | 10% |
Unknown | 7 | 24% |