| Title |
Ion–protein interactions of a potassium ion channel studied by attenuated total reflection Fourier transform infrared spectroscopy
|
|---|---|
| Published in |
Biophysical Reviews, November 2017
|
| DOI | 10.1007/s12551-017-0337-8 |
| Pubmed ID | |
| Authors |
Yuji Furutani |
| Abstract |
An understanding of ion-protein interactions is key to a better understanding of the molecular mechanisms of proteins, such as enzymes, ion channels, and ion pumps. A potassium ion channel, KcsA, has been extensively studied in terms of ion selectivity. Alkali metal cations in the selectivity filter were visualized by X-ray crystallography. Infrared spectroscopy has an intrinsically higher structural sensitivity due to frequency changes in molecular vibrations interacting with different ions. In this review article, I attempt to summarize ion-exchange-induced differences in Fourier transform infrared spectroscopy, as applied to KcsA, to explain how this method can be utilized to study ion-protein interactions in the KcsA selectivity filter. A band at 1680 cm(-1) in the amide I region would be a marker band for the ion occupancy of K(+), Rb(+), and Cs(+) in the filter. The band at 1627 cm(-1) observed in both Na(+) and Li(+) conditions suggests that the selectivity filter similarly interacts with these ions. In addition to the structural information, the results show that the titration of K(+) ions provides quantitative information on the ion affinity of the selectivity filter. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 23% |
| Researcher | 3 | 23% |
| Student > Ph. D. Student | 3 | 23% |
| Student > Doctoral Student | 2 | 15% |
| Student > Master | 1 | 8% |
| Other | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 23% |
| Chemistry | 3 | 23% |
| Nursing and Health Professions | 2 | 15% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Physics and Astronomy | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |
Attention Score in Context
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#20,452,930
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Outputs from Biophysical Reviews
#704
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Outputs of similar age from Biophysical Reviews
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