| Title |
Comparison of Chondroitin Sulfate and Hyaluronic Acid Doped Conductive Polypyrrole Films for Adipose Stem Cells
|
|---|---|
| Published in |
Annals of Biomedical Engineering, May 2014
|
| DOI | 10.1007/s10439-014-1023-7 |
| Pubmed ID | |
| Authors |
Miina Björninen, Aliisa Siljander, Jani Pelto, Jari Hyttinen, Minna Kellomäki, Susanna Miettinen, Riitta Seppänen, Suvi Haimi |
| Abstract |
Polypyrrole (PPy) is a conductive polymer that has aroused interest due to its biocompatibility with several cell types and high tailorability as an electroconductive scaffold coating. This study compares the effect of hyaluronic acid (HA) and chondroitin sulfate (CS) doped PPy films on human adipose stem cells (hASCs) under electrical stimulation. The PPy films were synthetized electrochemically. The surface morphology of PPy-HA and PPy-CS was characterized by an atomic force microscope. A pulsed biphasic electric current (BEC) was applied via PPy films non-stimulated samples acting as controls. Viability, attachment, proliferation and osteogenic differentiation of hASCs were evaluated by live/dead staining, DNA content, Alkaline phosphatase activity and mineralization assays. Human ASCs grew as a homogenous cell sheet on PPy-CS surfaces, whereas on PPy-HA cells clustered into small spherical structures. PPy-CS supported hASC proliferation significantly better than PPy-HA at the 7 day time point. Both substrates equally triggered early osteogenic differentiation of hASCs, although mineralization was significantly induced on PPy-CS compared to PPy-HA under BEC. These differences may be due to different surface morphologies originating from the CS and HA dopants. Our results suggest that PPy-CS in particular is a potential osteogenic scaffold coating for bone tissue engineering. |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 24% |
| Student > Master | 6 | 16% |
| Researcher | 5 | 13% |
| Student > Bachelor | 3 | 8% |
| Student > Doctoral Student | 2 | 5% |
| Other | 7 | 18% |
| Unknown | 6 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Materials Science | 7 | 18% |
| Biochemistry, Genetics and Molecular Biology | 6 | 16% |
| Engineering | 6 | 16% |
| Medicine and Dentistry | 4 | 11% |
| Chemistry | 2 | 5% |
| Other | 5 | 13% |
| Unknown | 8 | 21% |