Title |
Enhanced TH17 Responses in Patients with IL10 Receptor Deficiency and Infantile-onset IBD
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Published in |
Inflammatory Bowel Diseases, November 2017
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DOI | 10.1097/mib.0000000000001270 |
Pubmed ID | |
Authors |
Dror S. Shouval, Liza Konnikova, Alexandra E. Griffith, Sarah M. Wall, Amlan Biswas, Lael Werner, Moran Nunberg, Jochen Kammermeier, Jeremy A. Goettel, Rajsavi Anand, Hannah Chen, Batia Weiss, Jian Li, Anthony Loizides, Baruch Yerushalmi, Tadahiro Yanagi, Rita Beier, Laurie S. Conklin, Christen L. Ebens, Fernanda G. M. S. Santos, Mary Sherlock, Jeffery D. Goldsmith, Daniel Kotlarz, Sarah C. Glover, Neil Shah, Athos Bousvaros, Holm H. Uhlig, Aleixo M. Muise, Christoph Klein, Scott B. Snapper |
Abstract |
IL10 receptor (IL10R) deficiency causes severe infantile-onset inflammatory bowel disease. Intact IL10R-dependent signals have been shown to be important for innate and adaptive immune cell functions in mice. We have previously reported a key role of IL10 in the generation and function of human anti-inflammatory macrophages. Independent of innate immune cell defects, the aim of the current study was to determine the role of IL10R signaling in regulating human CD4 T-cell function. Peripheral blood mononuclear cells and intestinal biopsies cells were collected from IL10/IL10R-deficient patients and controls. Frequencies of CD4 T-cell subsets, naive T-cell proliferation, regulatory T cell (Treg)-mediated suppression, and Treg and TH17 generation were determined by flow cytometry. Transcriptional profiling was performed by NanoString and quantitative real-time polymerase chain reaction. RNA in situ hybridization was used to determine the quantities of various transcripts in intestinal mucosa. Analysis of 16 IL10- and IL10R-deficient patients demonstrated similar frequencies of peripheral blood and intestinal Tregs, compared with control subjects. In addition, in vitro Treg suppression of CD4 T-cell proliferation and generation of Treg were not dependent on IL10R signaling. However, IL10R-deficient T naive cells exhibited higher proliferative capacity, a strong TH17 signature, and an increase in polarization toward TH17 cells, compared with controls. Moreover, the frequency of TH17 cells was increased in the colon and ileum of IL10R-deficient patients. Finally, we show that stimulation of IL10R-deficient Tregs in the presence of IL1β leads to enhanced production of IL17A. IL10R signaling regulates TH17 polarization and T-cell proliferation in humans but is not required for the generation and in vitro suppression of Tregs. Therapies targeting the TH17 axis might be beneficial for IL10- and IL10R-deficient patients as a bridge to allogeneic hematopoietic stem cell transplantation. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 53 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 17% |
Researcher | 9 | 17% |
Student > Master | 9 | 17% |
Student > Bachelor | 5 | 9% |
Professor > Associate Professor | 4 | 8% |
Other | 6 | 11% |
Unknown | 11 | 21% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 13 | 25% |
Immunology and Microbiology | 8 | 15% |
Agricultural and Biological Sciences | 6 | 11% |
Biochemistry, Genetics and Molecular Biology | 4 | 8% |
Computer Science | 2 | 4% |
Other | 5 | 9% |
Unknown | 15 | 28% |