Title |
The ArlRS two-component system is a regulator of Staphylococcus aureus-induced endothelial cell damage
|
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Published in |
European Journal of Clinical Microbiology & Infectious Diseases, November 2017
|
DOI | 10.1007/s10096-017-3130-5 |
Pubmed ID | |
Authors |
Kati Seidl, Michèle Leemann, Annelies S. Zinkernagel |
Abstract |
Staphylococcus aureus endovascular infections retain a high morbidity and mortality despite antibiotics and supportive care. The destruction of endothelial cells (ECs) is a critical step in the pathogenesis of S. aureus endovascular infections. In order to better understand S. aureus-induced EC damage, we systematically screened a collection of two-component regulatory system mutants of methicillin-resistant S. aureus (MRSA) USA300 strain JE2 for damage induction in human umbilical vein ECs (HUVECs). This screen revealed that the two-component regulatory system ArlRS is required for maximum damage: arlRS inactivation leads to a > 70% reduction in damage. In a different genetic S. aureus background (RN6390, MSSA strain) arlRS inactivation had a smaller but also significant effect on EC damage. In both strains, the reduction in EC damage was accompanied by a significant reduction in internalization. In conclusion, we determined a novel role of ArlRS in S. aureus-induced EC damage, which will help to better understand the pathogenesis of S. aureus endovascular infection. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 21% |
Student > Ph. D. Student | 2 | 14% |
Student > Master | 2 | 14% |
Other | 1 | 7% |
Student > Postgraduate | 1 | 7% |
Other | 0 | 0% |
Unknown | 5 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 14% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Agricultural and Biological Sciences | 2 | 14% |
Immunology and Microbiology | 2 | 14% |
Chemistry | 1 | 7% |
Other | 0 | 0% |
Unknown | 5 | 36% |