You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
ATM Is Required for the Prolactin-Induced HSP90-Mediated Increase in Cellular Viability and Clonogenic Growth After DNA Damage
|
|---|---|
| Published in |
Molecular Endocrinology, November 2017
|
| DOI | 10.1210/en.2017-00652 |
| Pubmed ID | |
| Authors |
Ödül Karayazi Atici, Anna Urbanska, Sesha Gopal Gopinathan, Florence Boutillon, Vincent Goffin, Carrie S Shemanko |
| Abstract |
Prolactin acts as a survival factor for breast cancer cells, but the prolactin signaling pathway and the mechanism is unknown. Previously, we identified the master chaperone, heat shock protein 90α (HSP90α), as a prolactin-Janus-Kinase-(JAK2)-signal-transducer-and-activator-of-transcription-5-(STAT5) target gene involved in survival, and here we investigated the role of HSP90 in the mechanism of prolactin-induced viability in response to DNA damage. The ataxia-telangiectasia mutated kinase protein (ATM) plays a critical role in the cellular response to double strand DNA damage. Prolactin increased viability of breast cancer cells treated with doxorubicin or etoposide. The increase in cellular resistance is specific to the prolactin receptor, as the prolactin receptor antagonist, Δ1-9-G129R-hPRL, prevented the increase in viability. Two different HSP90 inhibitors, 17-allylamino-17-demethoxy geldanamycin and BIIB021, reduced the prolactin-mediated increase in cell viability of doxorubicin treated cells, and led to a decrease in JAK2, ATM and phospho-ATM protein levels. Inhibitors of JAK2 (G6) and ATM (KU55933) abolished the prolactin-mediated increase in cell viability of DNA damaged cells, supporting the involvement of each, as well as the cross-talk of ATM with the prolactin pathway in the context of DNA damage. Drug synergism was detected between the ATM inhibitor, KU55933, and doxorubicin, and also between the HSP90 inhibitor, BIIB021, and doxorubicin. Short interfering RNA, directed against ATM, prevented the PRL-mediated increase in cell survival in both 2D and 3D collagen gel cultures, and in clonogenic cell survival, after doxorubicin treatment. Our results indicate that ATM contributes to the prolactin-JAK2-STAT5-HSP90 pathway in mediating cellular resistance to DNA damaging agents. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 2 | 67% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 27% |
| Student > Bachelor | 2 | 13% |
| Student > Doctoral Student | 1 | 7% |
| Other | 1 | 7% |
| Lecturer | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 5 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 53% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Unknown | 6 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2021.
All research outputs
#15,097,241
of 25,382,440 outputs
Outputs from Molecular Endocrinology
#7,459
of 9,959 outputs
Outputs of similar age
#229,338
of 446,214 outputs
Outputs of similar age from Molecular Endocrinology
#28
of 72 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,959 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 446,214 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 72 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.