| Title |
LC-MS/MS imaging with thermal film-based laser microdissection
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, November 2017
|
| DOI | 10.1007/s00216-017-0739-2 |
| Pubmed ID | |
| Authors |
Michiko Oya, Hiromi Suzuki, Andrea Roxanne J. Anas, Koichi Oishi, Kenji Ono, Shun Yamaguchi, Megumi Eguchi, Makoto Sawada |
| Abstract |
Mass spectrometry (MS) imaging is a useful tool for direct and simultaneous visualization of specific molecules. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to evaluate the abundance of molecules in tissues using sample homogenates. To date, however, LC-MS/MS has not been utilized as an imaging tool because spatial information is lost during sample preparation. Here we report a new approach for LC-MS/MS imaging using a thermal film-based laser microdissection (LMD) technique. To isolate tissue spots, our LMD system uses a 808-nm near infrared laser, the diameter of which can be freely changed from 2.7 to 500 μm; for imaging purposes in this study, the diameter was fixed at 40 μm, allowing acquisition of LC-MS/MS images at a 40-μm resolution. The isolated spots are arranged on a thermal film at 4.5-mm intervals, corresponding to the well spacing on a 384-well plate. Each tissue spot is handled on the film in such a manner as to maintain its spatial information, allowing it to be extracted separately in its individual well. Using analytical LC-MS/MS in combination with the spatial information of each sample, we can reconstruct LC-MS/MS images. With this imaging technique, we successfully obtained the distributions of pilocarpine, glutamate, γ-aminobutyric acid, acetylcholine, and choline in a cross-section of mouse hippocampus. The protocol we established in this study is applicable to revealing the neurochemistry of pilocarpine model of epilepsy. Our system has a wide range of uses in fields such as biology, pharmacology, pathology, and neuroscience. Graphical abstract Schematic Indication of LMD-LC-MS/MS imaging. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 25% |
| United States | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 50% |
| Members of the public | 1 | 25% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 20% |
| Other | 4 | 16% |
| Student > Ph. D. Student | 3 | 12% |
| Unspecified | 1 | 4% |
| Lecturer | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 9 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 5 | 20% |
| Biochemistry, Genetics and Molecular Biology | 3 | 12% |
| Unspecified | 2 | 8% |
| Agricultural and Biological Sciences | 2 | 8% |
| Chemistry | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 9 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2018.
All research outputs
#17,292,294
of 25,382,440 outputs
Outputs from Analytical & Bioanalytical Chemistry
#5,671
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Outputs of similar age
#280,331
of 446,708 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#73
of 160 outputs
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