| Title |
Targeted Green-Red Photoconversion of EosFP, a Fluorescent Marker Protein
|
|---|---|
| Published in |
Journal of Biological Physics, December 2005
|
| DOI | 10.1007/s10867-005-0174-z |
| Pubmed ID | |
| Authors |
Sergey Ivanchenko, Carlheinz Röcker, Franz Oswald, Jörg Wiedenmann, G. Ulrich Nienhaus |
| Abstract |
EosFP is a novel fluorescent protein from the stony coral Lobophyllia hemprichii. Its gene was cloned in Escherichia coli to express the tetrameric wild-type protein. The protein emits strong green fluorescence (516 nm) that shifts toward red (581 nm) upon near-ultraviolet irradiation at ∼390 nm due to a photo-induced modification that involves a break in the peptide backbone next to the chromophore. Using site-directed mutagenesis, dimeric (d1EosFP, d2EosFP) and monomeric (mEosFP) variants were produced with essentially unaltered spectroscopic properties. Here we present a spectroscopic characterization of EosFP and its variants, including room- and low-temperature spectra, fluorescence lifetime determinations, two-photon excitation and two-photon photoconversion. Furthermore, by transfection of a human cancer (HeLa) cell with a fusion construct of a mitochondrial targeting sequence and d2EosFP, we demonstrate how localized photoconversion of EosFP can be employed for resolving intracellular processes. |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 54 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 20% |
| Student > Bachelor | 11 | 20% |
| Researcher | 8 | 15% |
| Student > Doctoral Student | 5 | 9% |
| Student > Master | 5 | 9% |
| Other | 7 | 13% |
| Unknown | 7 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 31% |
| Biochemistry, Genetics and Molecular Biology | 7 | 13% |
| Chemistry | 7 | 13% |
| Medicine and Dentistry | 4 | 7% |
| Physics and Astronomy | 3 | 6% |
| Other | 9 | 17% |
| Unknown | 7 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2020.
All research outputs
#7,542,164
of 23,009,818 outputs
Outputs from Journal of Biological Physics
#58
of 298 outputs
Outputs of similar age
#38,270
of 147,362 outputs
Outputs of similar age from Journal of Biological Physics
#2
of 4 outputs
Altmetric has tracked 23,009,818 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 298 research outputs from this source. They receive a mean Attention Score of 2.6. This one has gotten more attention than average, scoring higher than 65% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 147,362 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.