| Title |
Isolation and genotyping of potentially pathogenic Acanthamoeba and Naegleria species from tap-water sources in Osaka, Japan
|
|---|---|
| Published in |
Parasitology Research, June 2009
|
| DOI | 10.1007/s00436-009-1528-4 |
| Pubmed ID | |
| Authors |
Akiko Edagawa, Akio Kimura, Takako Kawabuchi-Kurata, Yasuhiro Kusuhara, Panagiotis Karanis |
| Abstract |
Here, we carried out a survey to determine the prevalence of free-living amoebae (FLA) in tap-water sources from rivers and water treatment plants located in Osaka Prefecture, Japan. A total of 374 raw water samples were collected from 113 sampling points. The samples were filtrated and transferred to non-nutrient agar plates seeded with a heat-killed suspension of Escherichia coli and incubated for 2 to 7 days at 30 degrees C or 42 degrees C. The plates were examined by microscopy to morphologically identify FLA families, and polymerase chain reaction and sequence analysis were then performed to define the species of the detected Naegleria and Acanthamoeba isolates. A total of 257 of 374 samples (68.7%) were positive for FLA by microscopy, and among these there were 800 FLA isolates, including Acanthamoeba and Naegleria species. Sequence analysis identified five Acanthamoeba spp. isolates of the known pathogenic T4 genotype and 43 Naegleria australiensis isolates, a reported pathogen to mice and also of concern as a potential pathogen to humans. Our results suggest a wide distribution of FLA, including potential pathogenic species, in tap-water sources of western Japan. |
Mendeley readers
The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 2% |
| Unknown | 65 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 12 | 18% |
| Researcher | 11 | 17% |
| Student > Bachelor | 10 | 15% |
| Student > Ph. D. Student | 9 | 14% |
| Student > Doctoral Student | 5 | 8% |
| Other | 8 | 12% |
| Unknown | 11 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 22 | 33% |
| Medicine and Dentistry | 10 | 15% |
| Immunology and Microbiology | 7 | 11% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Environmental Science | 5 | 8% |
| Other | 2 | 3% |
| Unknown | 15 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 March 2024.
All research outputs
#7,204,796
of 22,774,233 outputs
Outputs from Parasitology Research
#570
of 3,781 outputs
Outputs of similar age
#36,093
of 109,255 outputs
Outputs of similar age from Parasitology Research
#5
of 19 outputs
Altmetric has tracked 22,774,233 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 3,781 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 109,255 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.