↓ Skip to main content

An epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator

Overview of attention for article published in Nature Communications, December 2014
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (85th percentile)

Mentioned by

news
5 news outlets
blogs
2 blogs
twitter
20 X users
weibo
1 weibo user
facebook
1 Facebook page

Citations

dimensions_citation
112 Dimensions

Readers on

mendeley
266 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
An epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator
Published in
Nature Communications, December 2014
DOI 10.1038/ncomms6619
Pubmed ID
Authors

Dong-Sung Lee, Jong-Yeon Shin, Peter D. Tonge, Mira C. Puri, Seungbok Lee, Hansoo Park, Won-Chul Lee, Samer M. I. Hussein, Thomas Bleazard, Ji-Young Yun, Jihye Kim, Mira Li, Nicole Cloonan, David Wood, Jennifer L. Clancy, Rowland Mosbergen, Jae-Hyuk Yi, Kap-Seok Yang, Hyungtae Kim, Hwanseok Rhee, Christine A. Wells, Thomas Preiss, Sean M. Grimmond, Ian M. Rogers, Andras Nagy, Jeong-Sun Seo

Abstract

Reprogramming of somatic cells to induced pluripotent stem cells involves a dynamic rearrangement of the epigenetic landscape. To characterize this epigenomic roadmap, we have performed MethylC-seq, ChIP-seq (H3K4/K27/K36me3) and RNA-Seq on samples taken at several time points during murine secondary reprogramming as part of Project Grandiose. We find that DNA methylation gain during reprogramming occurs gradually, while loss is achieved only at the ESC-like state. Binding sites of activated factors exhibit focal demethylation during reprogramming, while ESC-like pluripotent cells are distinguished by extension of demethylation to the wider neighbourhood. We observed that genes with CpG-rich promoters demonstrate stable low methylation and strong engagement of histone marks, whereas genes with CpG-poor promoters are safeguarded by methylation. Such DNA methylation-driven control is the key to the regulation of ESC-pluripotency genes, including Dppa4, Dppa5a and Esrrb. These results reveal the crucial role that DNA methylation plays as an epigenetic switch driving somatic cells to pluripotency.

X Demographics

X Demographics

The data shown below were collected from the profiles of 20 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 266 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 <1%
United States 2 <1%
France 1 <1%
Australia 1 <1%
United Kingdom 1 <1%
Switzerland 1 <1%
Spain 1 <1%
China 1 <1%
Unknown 256 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 69 26%
Researcher 63 24%
Student > Master 26 10%
Student > Bachelor 18 7%
Professor 17 6%
Other 43 16%
Unknown 30 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 108 41%
Biochemistry, Genetics and Molecular Biology 76 29%
Medicine and Dentistry 12 5%
Engineering 10 4%
Computer Science 6 2%
Other 24 9%
Unknown 30 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 66. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 December 2015.
All research outputs
#615,043
of 24,525,936 outputs
Outputs from Nature Communications
#10,644
of 52,815 outputs
Outputs of similar age
#7,800
of 371,189 outputs
Outputs of similar age from Nature Communications
#111
of 750 outputs
Altmetric has tracked 24,525,936 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 52,815 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 56.1. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 371,189 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 750 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.