| Title |
Novel comprehensive multidimensional liquid chromatography approach for elucidation of the microbosphere of shikimate-producing Escherichia coli SP1.1/pKD15.071 strain
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, November 2017
|
| DOI | 10.1007/s00216-017-0744-5 |
| Pubmed ID | |
| Authors |
Francesco Cacciola, Domenica Mangraviti, Francesca Rigano, Paola Donato, Paola Dugo, Luigi Mondello, Hernan J. Cortes |
| Abstract |
Shikimic acid is a intermediate of aromatic amino acid biosynthesis and the preferred starting material for production of the most commonly prescribed anti-influenza drug, Tamiflu. Its six-membered carbocyclic ring is adorned with several chiral centers and various functionalities, making shikimic acid a valuable chiral synthon. When microbially-produced, in addition to shikimic acid, numerous other metabolites are exported out of the cytoplasm and accumulate in the culture medium. This extracellular matrix of metabolites is referred to as the microbosphere. Due to the high sample complexity, in this study, the microbosphere of shikimate-producing Escherichia coli SP1.1/pKD15.071 was analyzed by liquid chromatography and comprehensive two-dimensional liquid chromatography coupled to photodiode array and mass spectrometry detection. GC analysis of the trimethylsilyl derivatives was also carried out in order to support the elucidation of the selected metabolites in the microbosphere. The elucidation of the metabolic fraction of this bacterial strain might be of valid aid for improving, through genetic changes, the concentration and yield of shikimic acid synthesized from glucose. Graphical abstract. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 24% |
| Researcher | 2 | 12% |
| Student > Postgraduate | 2 | 12% |
| Professor > Associate Professor | 2 | 12% |
| Student > Bachelor | 1 | 6% |
| Other | 2 | 12% |
| Unknown | 4 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 8 | 47% |
| Environmental Science | 1 | 6% |
| Agricultural and Biological Sciences | 1 | 6% |
| Neuroscience | 1 | 6% |
| Unknown | 6 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 May 2018.
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#20,663,600
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Outputs from Analytical & Bioanalytical Chemistry
#6,602
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#339,327
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#95
of 170 outputs
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