| Title |
Performances of two different panfungal PCRs to detect mould DNA in formalin-fixed paraffin-embedded tissue: what are the limiting factors?
|
|---|---|
| Published in |
BMC Infectious Diseases, December 2014
|
| DOI | 10.1186/s12879-014-0692-z |
| Pubmed ID | |
| Authors |
B Babouee Flury, M Weisser, S Savič Prince, L Bubendorf, M Battegay, R Frei, D Goldenberger |
| Abstract |
Detection of fungal DNA from formalin-fixed, paraffin-embedded (FFPE) tissue is challenging due to degradation of DNA and presence of PCR inhibitors in these samples. We analyzed FFPE samples of 26 patients by panfungal PCR and compared the results to the composite diagnosis according to the European Organization for Research and Treatment of Cancer (EORTC) criteria. Additionally we analyzed the quality of human and fungal DNA and their level of age-dependent degradation, as well as the existence of PCR inhibition in these tissue samples. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 2% |
| Unknown | 51 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 7 | 13% |
| Researcher | 7 | 13% |
| Student > Ph. D. Student | 6 | 12% |
| Student > Postgraduate | 6 | 12% |
| Student > Master | 5 | 10% |
| Other | 11 | 21% |
| Unknown | 10 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 23% |
| Medicine and Dentistry | 11 | 21% |
| Immunology and Microbiology | 4 | 8% |
| Biochemistry, Genetics and Molecular Biology | 3 | 6% |
| Engineering | 3 | 6% |
| Other | 6 | 12% |
| Unknown | 13 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2014.
All research outputs
#18,386,678
of 22,774,233 outputs
Outputs from BMC Infectious Diseases
#5,593
of 7,669 outputs
Outputs of similar age
#255,983
of 353,309 outputs
Outputs of similar age from BMC Infectious Diseases
#133
of 192 outputs
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We're also able to compare this research output to 192 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.