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Mendeley readers
| Title |
Crystallization and preliminary X‐ray crystallographic analysis of 3‐deoxy‐d‐arabino‐heptulosonate‐7‐phosphate synthase from Mycobacterium tuberculosis
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|---|---|
| Published in |
Acta Crystallographica Section F: Structural Biology Communications, March 2005
|
| DOI | 10.1107/s1744309105007931 |
| Pubmed ID | |
| Authors |
Celia J Webby, J Shaun Lott, Heather M Baker, Edward N Baker, Emily J Parker |
| Abstract |
The enzymes of the shikimate pathway are attractive targets for new-generation antimicrobial agents. The first step of this pathway is catalysed by 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAH7P) synthase and involves the condensation of phosphoenolpyruvate (PEP) and erythrose 4-phosphate (E4P) to form DAH7P. DAH7P synthases have been classified into two apparently evolutionarily unrelated types and whereas structural data have been obtained for the type I DAH7P synthases, no structural information is available for their type II counterparts. The type II DAH7P synthase from Mycobacterium tuberculosis was co-expressed as native and selenomethionine-substituted protein with the Escherichia coli chaperonins GroEL and GroES in E. coli, purified and crystallized. Native crystals of M. tuberculosis DAH7P synthase belong to space group P3(1)21 or P3(2)21 and diffract to 2.5 A, with unit-cell parameters a = b = 203.61, c = 66.39 A. There are either two or three molecules in the asymmetric unit. Multiwavelength anomalous diffraction (MAD) phasing using selenomethionine-substituted protein is currently under way. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Czechia | 2 | 17% |
| Mexico | 1 | 8% |
| Unknown | 9 | 75% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 25% |
| Student > Master | 3 | 25% |
| Researcher | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Unknown | 4 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 5 | 42% |
| Agricultural and Biological Sciences | 3 | 25% |
| Unknown | 4 | 33% |