Title |
A Novel Flow Cytometric Method To Assess Inflammasome Formation
|
---|---|
Published in |
The Journal of Immunology, January 2015
|
DOI | 10.4049/jimmunol.1401110 |
Pubmed ID | |
Authors |
David P Sester, Sara J Thygesen, Vitaliya Sagulenko, Parimala R Vajjhala, Jasmyn A Cridland, Nazarii Vitak, Kaiwen W Chen, Geoffrey W Osborne, Kate Schroder, Katryn J Stacey |
Abstract |
Inflammasomes are large protein complexes induced by a wide range of microbial, stress, and environmental stimuli that function to induce cell death and inflammatory cytokine processing. Formation of an inflammasome involves dramatic relocalization of the inflammasome adapter protein apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) into a single speck. We have developed a flow cytometric assay for inflammasome formation, time of flight inflammasome evaluation, which detects the change in ASC distribution within the cell. The transit of ASC into the speck is detected by a decreased width or increased height of the pulse of emitted fluorescence. This assay can be used to quantify native inflammasome formation in subsets of mixed cell populations ex vivo. It can also provide a rapid and sensitive technique for investigating molecular interactions in inflammasome formation, by comparison of wild-type and mutant proteins in inflammasome reconstitution experiments. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Australia | 1 | 20% |
United Kingdom | 1 | 20% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 4 | 80% |
Science communicators (journalists, bloggers, editors) | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | <1% |
Norway | 1 | <1% |
Australia | 1 | <1% |
United Kingdom | 1 | <1% |
Spain | 1 | <1% |
Japan | 1 | <1% |
United States | 1 | <1% |
Unknown | 155 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 36 | 22% |
Student > Ph. D. Student | 33 | 20% |
Student > Bachelor | 15 | 9% |
Student > Master | 14 | 9% |
Professor > Associate Professor | 10 | 6% |
Other | 28 | 17% |
Unknown | 26 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 47 | 29% |
Immunology and Microbiology | 33 | 20% |
Medicine and Dentistry | 25 | 15% |
Biochemistry, Genetics and Molecular Biology | 18 | 11% |
Neuroscience | 3 | 2% |
Other | 5 | 3% |
Unknown | 31 | 19% |