| Title |
Metabolic effects of physiological levels of caffeine in myotubes
|
|---|---|
| Published in |
Journal of Physiology and Biochemistry, December 2017
|
| DOI | 10.1007/s13105-017-0601-1 |
| Pubmed ID | |
| Authors |
Jamie K. Schnuck, Lacey M. Gould, Hailey A. Parry, Michele A. Johnson, Nicholas P. Gannon, Kyle L. Sunderland, Roger A. Vaughan |
| Abstract |
Caffeine has been shown to stimulate multiple major regulators of cell energetics including AMP-activated protein kinase (AMPK) and Ca2+/calmodulin-dependent protein kinase II (CaMKII). Additionally, caffeine induces peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and mitochondrial biogenesis. While caffeine enhances oxidative metabolism, experimental concentrations often exceed physiologically attainable concentrations through diet. This work measured the effects of low-level caffeine on cellular metabolism and gene expression in myotubes, as well as the dependence of caffeine's effects on the nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARβ/δ). C2C12 myotubes were treated with various doses of caffeine for up to 24 h. Gene and protein expression were measured via qRT-PCR and Western blot, respectively. Cellular metabolism was determined via oxygen consumption and extracellular acidification rate. Caffeine significantly induced regulators of mitochondrial biogenesis and oxidative metabolism. Mitochondrial staining was suppressed in PPARβ/δ-inhibited cells which was rescued by concurrent caffeine treatment. Caffeine-treated cells also displayed elevated peak oxidative metabolism which was partially abolished following PPARβ/δ inhibition. Similar to past observations, glucose uptake and GLUT4 content were elevated in caffeine-treated cells, however, glycolytic metabolism was unaltered following caffeine treatment. Physiological levels of caffeine appear to enhance cell metabolism through mechanisms partially dependent on PPARβ/δ. |
X Demographics
The data shown below were collected from the profiles of 18 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 11% |
| France | 1 | 6% |
| United States | 1 | 6% |
| United Kingdom | 1 | 6% |
| Australia | 1 | 6% |
| Unknown | 12 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 16 | 89% |
| Scientists | 1 | 6% |
| Practitioners (doctors, other healthcare professionals) | 1 | 6% |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 46 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 22% |
| Student > Master | 7 | 15% |
| Other | 3 | 7% |
| Student > Bachelor | 3 | 7% |
| Student > Doctoral Student | 2 | 4% |
| Other | 5 | 11% |
| Unknown | 16 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 28% |
| Sports and Recreations | 5 | 11% |
| Agricultural and Biological Sciences | 3 | 7% |
| Environmental Science | 1 | 2% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 6 | 13% |
| Unknown | 17 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 December 2021.
All research outputs
#2,787,166
of 25,124,631 outputs
Outputs from Journal of Physiology and Biochemistry
#37
of 599 outputs
Outputs of similar age
#59,519
of 451,400 outputs
Outputs of similar age from Journal of Physiology and Biochemistry
#2
of 4 outputs
Altmetric has tracked 25,124,631 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 599 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 451,400 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.