| Title |
MALDI Mass Spectrometry Imaging for Evaluation of Therapeutics in Colorectal Tumor Organoids
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, December 2017
|
| DOI | 10.1007/s13361-017-1851-4 |
| Pubmed ID | |
| Authors |
Xin Liu, Colin Flinders, Shannon M. Mumenthaler, Amanda B. Hummon |
| Abstract |
Patient-derived colorectal tumor organoids (CTOs) closely recapitulate the complex morphological, phenotypic, and genetic features observed in in vivo tumors. Therefore, evaluation of drug distribution and metabolism in this model system can provide valuable information to predict the clinical outcome of a therapeutic response in individual patients. In this report, we applied matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to examine the spatial distribution of the drug irinotecan and its metabolites in CTOs from two patients. Irinotecan is a prodrug and is often prescribed as part of therapeutic regimes for patients with advanced colorectal cancer. Irinotecan shows a time-dependent and concentration-dependent permeability and metabolism in the CTOs. More interestingly, the active metabolite SN-38 does not co-localize well with the parent drug irinotecan and the inactive metabolite SN-38G. The phenotypic effect of irinotecan metabolism was also confirmed by a viability study showing significantly reduced proliferation in the drug treated CTOs. MALDI-MSI can be used to investigate various pharmaceutical compounds in CTOs derived from different patients. By analyzing multiple CTOs from a patient, this method could be used to predict patient-specific drug responses and help to improve personalized dosing regimens. Graphical Abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 33% |
| United States | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 76 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 24% |
| Researcher | 10 | 13% |
| Student > Bachelor | 9 | 12% |
| Student > Doctoral Student | 7 | 9% |
| Student > Master | 6 | 8% |
| Other | 7 | 9% |
| Unknown | 19 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 14 | 18% |
| Biochemistry, Genetics and Molecular Biology | 13 | 17% |
| Agricultural and Biological Sciences | 7 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 7% |
| Engineering | 4 | 5% |
| Other | 10 | 13% |
| Unknown | 23 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 March 2018.
All research outputs
#16,673,062
of 25,508,813 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#2,588
of 3,851 outputs
Outputs of similar age
#264,476
of 446,625 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#23
of 46 outputs
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So far Altmetric has tracked 3,851 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
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