Title |
Cell density impacts on Candida glabrata survival in hypo-osmotic stress
|
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Published in |
FEMS Yeast Research, December 2013
|
DOI | 10.1111/1567-1364.12122 |
Pubmed ID | |
Authors |
Hala Almshawit, Dodie Pouniotis, Ian Macreadie |
Abstract |
Candida glabrata cells suspended in water are under hypo-osmotic stress and undergo cell death in 1-2 days, unless they are at a density of more than 10(5 ) CFU mL(-1) . The dying cells exhibit FITC-annexin V staining, indicative of programmed cell death (apoptosis). In a higher cell density, cells are protected and survive at least for 4 days. Filtrates from cells at high density can protect those at lower density, indicating that cells release substances, amounting to c. 5 mg L(-1) of cell suspension, that protect each other against hypo-osmotic stress. In a concentrated form, the released materials can support growth, indicating that the protective material includes carbon and nitrogen sources, as well as vitamins that are required by C. glabrata for growth. We conclude that cell death from hypo-osmotic stress can be alleviated by small amounts of nutrients. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 19% |
Student > Master | 4 | 19% |
Researcher | 4 | 19% |
Student > Bachelor | 3 | 14% |
Unknown | 6 | 29% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 29% |
Medicine and Dentistry | 2 | 10% |
Agricultural and Biological Sciences | 2 | 10% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Nursing and Health Professions | 1 | 5% |
Other | 2 | 10% |
Unknown | 7 | 33% |