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No Evidence of Pathogenic Involvement of Cathelicidins in Patient Cohorts and Mouse Models of Lupus and Arthritis

Overview of attention for article published in PLOS ONE, December 2014
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Title
No Evidence of Pathogenic Involvement of Cathelicidins in Patient Cohorts and Mouse Models of Lupus and Arthritis
Published in
PLOS ONE, December 2014
DOI 10.1371/journal.pone.0115474
Pubmed ID
Authors

D. Kienhöfer, J. Hahn, I. Schubert, C. Reinwald, N. Ipseiz, S. C. Lang, È. Bosch Borràs, K. Amann, C. Sjöwall, A. E. Barron, A. J. Hueber, B. Agerberth, G. Schett, M. H. Hoffmann

Abstract

Apart from their role in the immune defence against pathogens evidence of a role of antimicrobial peptides (AMPs) in autoimmune diseases has accumulated in the past years. The aim of this project was to examine the functional impact of the human cathelicidin LL-37 and the mouse cathelicidin-related AMP (CRAMP) on the pathogenesis of lupus and arthritis. Serum LL-37 and anti-LL-37 levels were measured by ELISA in healthy donors and patients with Systemic Lupus Erythematosus (SLE) and Rheumatoid arthritis (RA). Pristane-induced lupus was induced in female wild type (WT) and cathelicidin-deficient (CRAMP-/-) mice. Serum levels of anti-Sm/RNP, anti-dsDNA, and anti-histone were determined via ELISA, cytokines in sera and peritoneal lavages were measured via Multiplex. Expression of Interferon I stimulated genes (ISG) was determined by real-time PCR. Collagen-induced arthritis (CIA) was induced in male WT and CRAMP-/- mice and arthritis severity was visually scored and analysed histomorphometrically by OsteoMeasure software. Serum levels of anti-LL-37 were higher in SLE-patients compared to healthy donors or patients with RA. However, no correlation to markers of disease activity or organ involvement was observed. No significant differences of autoantibody or cytokine/chemokine levels, or of expression of ISGs were observed between WT and CRAMP-/- mice after pristane-injection. Furthermore, lung and kidney pathology did not differ in the absence of CRAMP. Incidence and severity of CIA and histological parameters (inflammation, cartilage degradation, and bone erosion) were not different in WT and CRAMP-/- mice. Although cathelicidins are upregulated in mouse models of lupus and arthritis, cathelicidin-deficiency did not persistently affect the diseases. Also in patients with SLE, autoantibodies against cathelicidins did not correlate with disease manifestation. Reactivity against cathelicidins in lupus and arthritis could thus be an epiphenomenon caused by extensive overexpression in blood and affected tissues. In addition, other cationic AMPs could functionally compensate for the deficiency of cathelicidins.

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The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 48 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 21%
Student > Master 9 19%
Student > Ph. D. Student 8 17%
Student > Bachelor 4 8%
Student > Postgraduate 3 6%
Other 6 13%
Unknown 8 17%
Readers by discipline Count As %
Medicine and Dentistry 17 35%
Immunology and Microbiology 8 17%
Agricultural and Biological Sciences 6 13%
Biochemistry, Genetics and Molecular Biology 5 10%
Mathematics 1 2%
Other 2 4%
Unknown 9 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 March 2016.
All research outputs
#20,247,117
of 22,775,504 outputs
Outputs from PLOS ONE
#173,463
of 194,340 outputs
Outputs of similar age
#295,563
of 352,836 outputs
Outputs of similar age from PLOS ONE
#2,776
of 3,402 outputs
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