| Title |
Biodegradation of hexachlorobenzene by a constructed microbial consortium
|
|---|---|
| Published in |
World Journal of Microbiology and Biotechnology, December 2014
|
| DOI | 10.1007/s11274-014-1789-7 |
| Pubmed ID | |
| Authors |
Da-Zhong Yan, Ling-Qi Mao, Cun-Zhi Li, Jun Liu |
| Abstract |
A consortium comprised of an engineered Escherichia coli DH5α and a natural pentachlorophenol (PCP) degrader, Sphingobium chlorophenolicum ATCC 39723, was assembled for degradation of hexachlorobenzene (HCB), a persistent organic pollutant. The engineered E. coli strain, harbouring a gene cassette (camA (+) camB (+) camC) that encodes the F87W/Y96F/L244A/V247L mutant of cytochrome P-450cam (CYP101), oxidised HCB to PCP. The resulting PCP was then further completely degraded by ATCC 39723. The results showed that almost 40 % of 4 μM HCB was degraded by the consortium at a rate of 0.033 nmol/mg (dry weight)/h over 24 h, accompanied by transient accumulation and immediate consumption of the intermediate PCP, detected by gas chromatography. In contrast, in the consortium comprised of Pseudomonas putida PaW340 harbouring camA (+) camB (+) camC and ATCC 39723, PCP accumulated in PaW340 cells but could not be further degraded, which may be due to a permeability barrier of Pseudomonas PaW340 for PCP transportation. The strategy of bacterial co-culture may provide an alternative approach for the bioremediation of HCB contamination. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 26% |
| Other | 4 | 17% |
| Student > Ph. D. Student | 3 | 13% |
| Researcher | 2 | 9% |
| Student > Master | 2 | 9% |
| Other | 1 | 4% |
| Unknown | 5 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 4 | 17% |
| Engineering | 4 | 17% |
| Biochemistry, Genetics and Molecular Biology | 3 | 13% |
| Agricultural and Biological Sciences | 3 | 13% |
| Business, Management and Accounting | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 6 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 October 2015.
All research outputs
#15,687,152
of 23,911,072 outputs
Outputs from World Journal of Microbiology and Biotechnology
#928
of 1,757 outputs
Outputs of similar age
#204,954
of 359,415 outputs
Outputs of similar age from World Journal of Microbiology and Biotechnology
#4
of 20 outputs
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So far Altmetric has tracked 1,757 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
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