Chemosensory cues signalling predators reliably stimulate innate defensive responses in rodents. Despite the well documented role of the amygdala in predator odor-induced fear, the relative contribution of the specific nuclei that comprise this structurally heterogeneous structure is conflicting. In an effort to clarify this we examined neural activity, via electrophysiological recordings, in amygdala subnuclei to controlled and repeated presentations of a predator odor; cat urine. Defensive behaviors, characterised by avoidance, decreased exploration and increased risk assessment were observed in adult male hooded Wistar rats (n=11) exposed to a cloth impregnated with cat urine. Electrophysiological recordings of the amygdala (777 multi-unit clusters) were subsequently obtained in freely-breathing anesthetised rats exposed to cat urine, distilled water and eugenol via an air-dilution olfactometer. Recorded units selectively responded to cat urine and frequencies of responses were distributed differently across amygdala nuclei; medial amygdala (MeA) demonstrated the greatest frequency of responses to cat urine (51.7%), followed by the baso-lateral and -medial nuclei (18.8%), and lastly the central amygdala (3.0%). Temporally, information transduction occurred primarily from the cortical and MeA (ventral divisions) to other amygdala nuclei. Interestingly, MeA subnuclei exhibited distinct firing patterns to predator urine, potentially revealing aspects of the underlying neurocircuitry of predator odor processing and defensiveness. These findings highlight the critical involvement of the MeA in processing olfactory cues signalling predator threat and converge with previous studies to indicate that amygdala regulation of predator-odor induced fear is restricted to a particular set of subnuclei which primarily include the MeA, particularly the ventral divisions.