| Title |
Nucleoprotein-based indirect enzyme-linked immunosorbent assay (indirect ELISA) for detecting antibodies specific to Ebola virus and Marbug virus
|
|---|---|
| Published in |
Virologica Sinica, December 2014
|
| DOI | 10.1007/s12250-014-3512-0 |
| Pubmed ID | |
| Authors |
Yi Huang, Youjie Zhu, Mengshi Yang, Zhenqing Zhang, Donglin Song, Zhiming Yuan |
| Abstract |
Full-length nucleoproteins from Ebola and Marburg viruses were expressed as His-tagged recombinant proteins in Escherichia coli and nucleoprotein-based enzyme-linked immunosorbent assays (ELISAs) were established for the detection of antibodies specific to Ebola and Marburg viruses. The ELISAs were evaluated by testing antisera collected from rabbit immunized with Ebola and Marburg virus nucleoproteins. Although little cross-reactivity of antibodies was observed in anti-Ebola virus nucleoprotein rabbit antisera, the highest reactions to immunoglobulin G (IgG) were uniformly detected against the nucleoprotein antigens of homologous viruses. We further evaluated the ELISA's ability to detect antibodies to Ebola and Marburg viruses using human sera samples collected from individuals passing through the Guangdong port of entry. With a threshold set at the mean plus three standard deviations of average optical densities of sera tested, the ELISA systems using these two recombinant nucleoproteins have good sensitivity and specificity. These results demonstrate the usefulness of ELISA for diagnostics as well as ecological and serosurvey studies of Ebola and Marburg virus infection. |
Mendeley readers
The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Australia | 1 | 2% |
| Unknown | 52 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 11 | 20% |
| Researcher | 11 | 20% |
| Student > Master | 10 | 19% |
| Student > Ph. D. Student | 7 | 13% |
| Librarian | 3 | 6% |
| Other | 6 | 11% |
| Unknown | 6 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 39% |
| Medicine and Dentistry | 7 | 13% |
| Biochemistry, Genetics and Molecular Biology | 5 | 9% |
| Nursing and Health Professions | 3 | 6% |
| Chemistry | 2 | 4% |
| Other | 7 | 13% |
| Unknown | 9 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 January 2015.
All research outputs
#17,712,179
of 22,776,824 outputs
Outputs from Virologica Sinica
#336
of 570 outputs
Outputs of similar age
#242,157
of 354,436 outputs
Outputs of similar age from Virologica Sinica
#9
of 12 outputs
Altmetric has tracked 22,776,824 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 570 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 12.1. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.