| Title |
UV Lamp as a Facile Ozone Source for Structural Analysis of Unsaturated Lipids Via Electrospray Ionization-Mass Spectrometry
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, December 2017
|
| DOI | 10.1007/s13361-017-1861-2 |
| Pubmed ID | |
| Authors |
Craig A. Stinson, Wenpeng Zhang, Yu Xia |
| Abstract |
Ozonolysis of alkene functional groups is a type of highly specific and effective chemical reaction, which has found increasing applications in structural analysis of unsaturated lipids via coupling with mass spectrometry (MS). In this work, we utilized a low-pressure mercury lamp (6 W) to initiate ozonolysis inside electrospray ionization (ESI) sources. By placing the lamp near a nanoESI emitter that partially transmits 185 nm ultraviolet (UV) emission from the lamp, dissolved dioxygen in the spray solution was converted into ozone, which subsequently cleaved the double bonds within fatty acyls of lipids. Solvent conditions, such as presence of water and acid solution pH, were found to be critical in optimizing ozonolysis yields. Fast (on seconds time scale) and efficient (50%-100% yield) ozonolysis was achieved for model unsaturated phospholipids and fatty acids with UV lamp-induced ozonolysis incorporated on a static and an infusion nanoESI source. The method was able to differentiate double bond location isomers and identify the geometry of the double bond based on yield. The analytical utility of UV lamp-induced ozonolysis was further demonstrated by implementation on a liquid chromatography (LC)-MS platform. Ozonolysis was effected in a flow microreactor that was made from ozone permeable tubing, so that ambient ozone produced by the lamp irradiation could diffuse into the reactor and induce online ozonolysis post-LC separation and before ESI-MS. Graphical Abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 46% |
| Student > Doctoral Student | 2 | 8% |
| Researcher | 2 | 8% |
| Professor | 1 | 4% |
| Student > Master | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 12 | 50% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Psychology | 1 | 4% |
| Arts and Humanities | 1 | 4% |
| Earth and Planetary Sciences | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 December 2017.
All research outputs
#19,951,180
of 25,382,440 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#2,946
of 3,835 outputs
Outputs of similar age
#320,105
of 443,738 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#29
of 45 outputs
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