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Genome-wide discovery of human splicing branchpoints

Overview of attention for article published in Genome Research, January 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (93rd percentile)
  • Good Attention Score compared to outputs of the same age and source (72nd percentile)

Mentioned by

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27 X users
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3 patents
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2 Facebook pages
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1 Google+ user

Citations

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197 Dimensions

Readers on

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293 Mendeley
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4 CiteULike
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Title
Genome-wide discovery of human splicing branchpoints
Published in
Genome Research, January 2015
DOI 10.1101/gr.182899.114
Pubmed ID
Authors

Tim R. Mercer, Michael B. Clark, Stacey B. Andersen, Marion E. Brunck, Wilfried Haerty, Joanna Crawford, Ryan J. Taft, Lars K. Nielsen, Marcel E. Dinger, John S. Mattick

Abstract

During the splicing reaction, the 5' intron end is joined to the branchpoint nucleotide, selecting the next exon to incorporate into the mature RNA and forming an intron lariat, which is excised. Despite a critical role in gene splicing, the locations and features of human splicing branchpoints are largely unknown. We use exoribonuclease digestion and targeted RNA-sequencing to enrich for sequences that traverse the lariat junction and, by split and inverted alignment, reveal the branchpoint. We identify 59,359 high-confidence human branchpoints in >10,000 genes, providing a first map of splicing branchpoints in the human genome. Branchpoints are predominantly adenosine, highly conserved, and closely distributed to the 3' splice site. Analysis of human branchpoints reveals numerous novel features, including distinct features of branchpoints for alternatively spliced exons and a family of conserved sequence motifs overlapping branchpoints we term B-boxes, which exhibit maximal nucleotide diversity while maintaining interactions with the keto-rich U2 snRNA. Different B-box motifs exhibit divergent usage in vertebrate lineages and associate with other splicing elements and distinct intron-exon architectures, suggesting integration within a broader regulatory splicing code. Lastly, although branchpoints are refractory to common mutational processes and genetic variation, mutations occurring at branchpoint nucleotides are enriched for disease associations.

X Demographics

X Demographics

The data shown below were collected from the profiles of 27 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 293 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 1%
United Kingdom 4 1%
Sweden 2 <1%
Hong Kong 1 <1%
Netherlands 1 <1%
China 1 <1%
Denmark 1 <1%
Japan 1 <1%
Spain 1 <1%
Other 0 0%
Unknown 277 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 81 28%
Researcher 62 21%
Student > Master 29 10%
Student > Bachelor 16 5%
Professor 13 4%
Other 41 14%
Unknown 51 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 106 36%
Biochemistry, Genetics and Molecular Biology 92 31%
Computer Science 9 3%
Medicine and Dentistry 8 3%
Neuroscience 5 2%
Other 19 6%
Unknown 54 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 21. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 July 2023.
All research outputs
#1,689,169
of 24,620,113 outputs
Outputs from Genome Research
#805
of 4,369 outputs
Outputs of similar age
#23,241
of 362,651 outputs
Outputs of similar age from Genome Research
#10
of 33 outputs
Altmetric has tracked 24,620,113 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,369 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.2. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 362,651 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.