| Title |
A novel fibrin-based artificial ovary prototype resembling human ovarian tissue in terms of architecture and rigidity
|
|---|---|
| Published in |
Journal of Assisted Reproduction and Genetics, December 2017
|
| DOI | 10.1007/s10815-017-1091-3 |
| Pubmed ID | |
| Authors |
Maria Costanza Chiti, Marie-Madeleine Dolmans, Lucie Mortiaux, Flanco Zhuge, Emna Ouni, Parinaz Asiabi Kohneh Shahri, Evelyne Van Ruymbeke, Sophie-Demoustier Champagne, Jacques Donnez, Christiani Andrade Amorim |
| Abstract |
The aim of this study is to optimize fibrin matrix composition in order to mimic human ovarian tissue architecture for human ovarian follicle encapsulation and grafting. Ultrastructure of fresh human ovarian cortex in age-related women (n = 3) and different fibrin formulations (F12.5/T1, F30/T50, F50/T50, F75/T75), rheology of fibrin matrices and histology of isolated and encapsulated human ovarian follicles in these matrices. Fresh human ovarian cortex showed a highly fibrous and structurally inhomogeneous architecture in three age-related patients, but the mean ± SD of fiber thickness (61.3 to 72.4 nm) was comparable between patients. When the fiber thickness of four different fibrin formulations was compared with human ovarian cortex, F50/T50 and F75/T75 showed similar fiber diameters to native tissue, while F12.5/T1 was significantly different (p value < 0.01). In addition, increased concentrations of fibrin exhibited enhanced storage modulus with F50/T50, resembling physiological ovarian rigidity. Excluding F12.5/T1 from further analysis, only three remaining fibrin matrices (F30/T50, F50/T50, F75/T75) were histologically investigated. For this, frozen-thawed fragments of human ovarian tissue collected from 22 patients were used to isolate ovarian follicles and encapsulate them in the three fibrin formulations. All three yielded similar follicle recovery and loss rates soon after encapsulation. Therefore, based on fiber thickness, porosity, and rigidity, we selected F50/T50 as the fibrin formulation that best mimics native tissue. Of all the different fibrin matrix concentrations tested, F50/T50 emerged as the combination of choice in terms of ultrastructure and rigidity, most closely resembling human ovarian cortex. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 25% |
| United States | 1 | 25% |
| Belgium | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 74 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 22% |
| Student > Bachelor | 8 | 11% |
| Student > Doctoral Student | 5 | 7% |
| Student > Master | 5 | 7% |
| Researcher | 5 | 7% |
| Other | 11 | 15% |
| Unknown | 24 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 10 | 14% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Medicine and Dentistry | 9 | 12% |
| Agricultural and Biological Sciences | 6 | 8% |
| Chemical Engineering | 2 | 3% |
| Other | 7 | 9% |
| Unknown | 30 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 73. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 July 2018.
All research outputs
#541,032
of 24,119,703 outputs
Outputs from Journal of Assisted Reproduction and Genetics
#20
of 1,697 outputs
Outputs of similar age
#12,971
of 446,846 outputs
Outputs of similar age from Journal of Assisted Reproduction and Genetics
#1
of 30 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,697 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one has done particularly well, scoring higher than 98% of its peers.
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We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.