| Title |
Engineering the robustness of Saccharomyces cerevisiae by introducing bifunctional glutathione synthase gene
|
|---|---|
| Published in |
Journal of Industrial Microbiology & Biotechnology, April 2015
|
| DOI | 10.1007/s10295-014-1573-6 |
| Pubmed ID | |
| Authors |
Zhiqi Qiu, Zujun Deng, Hongming Tan, Shining Zhou, Lixiang Cao |
| Abstract |
Robust, high-yielding Saccharomyces cerevisiae is highly desirable for cost-effective cellulosic ethanol production. In this study, the bifunctional glutathione (GSH) synthetase genes GCSGS at high copy number was integrated into ribosomal DNA of S. cerevisiae by Cre-LoxP system. Threefold higher GSH contents (54.9 μmol/g dry weight) accumulated in the engineered strain BY-G compared to the reference strain. Tolerance of BY-G to H2O2 (3 mM), temperature (40 °C), furfural (10 mM), hydroxymethylfurfural (HMF, 10 mM) and 0.5 mM Cd(2+) increased compared to reference strain. Twofold higher ethanol concentration was obtained by BY-G in simultaneous saccharification and fermentation of corn stover compared to the reference strain. The results showed that intracellular GSH content of S. cerevisiae has an influence on robustness. The strategy is used to engineer S. cerevisiae strains adaptive to a combination of tolerance to inhibitors and raised temperature that may occur in high solid simultaneous saccharification and fermentation of lignocellulosic feedstocks. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 2% |
| Canada | 1 | 2% |
| Unknown | 42 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 32% |
| Student > Ph. D. Student | 7 | 16% |
| Student > Postgraduate | 5 | 11% |
| Student > Bachelor | 2 | 5% |
| Other | 2 | 5% |
| Other | 5 | 11% |
| Unknown | 9 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 18 | 41% |
| Biochemistry, Genetics and Molecular Biology | 6 | 14% |
| Environmental Science | 2 | 5% |
| Chemistry | 2 | 5% |
| Chemical Engineering | 1 | 2% |
| Other | 5 | 11% |
| Unknown | 10 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 January 2015.
All research outputs
#22,759,802
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Outputs from Journal of Industrial Microbiology & Biotechnology
#1,453
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Outputs of similar age
#240,617
of 279,170 outputs
Outputs of similar age from Journal of Industrial Microbiology & Biotechnology
#24
of 35 outputs
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