Title |
Engineering genome-reduced Bacillus subtilis for acetoin production from xylose
|
---|---|
Published in |
Biotechnology Techniques, December 2017
|
DOI | 10.1007/s10529-017-2481-4 |
Pubmed ID | |
Authors |
Panpan Yan, Yuanqing Wu, Li Yang, Zhiwen Wang, Tao Chen |
Abstract |
To investigate the capacity of a genome-reduced Bacillus subtilis strain as chassis cell for acetoin production from xylose. To endow the genome-reduced Bacillus subtilis strain BSK814 with the ability to utilize xylose, we inserted a native xyl operon into its genome and deleted the araR gene. The resulting strain BSK814A2 produced 2.94 g acetoin/l from 10 g xylose/l, which was 39% higher than control strain BSK19A2. The deletion of the bdhA and acoA genes further improved xylose utilization efficiency and increased acetoin production to 3.71 g/l in BSK814A4. Finally, BSK814A4 produced up to 23.3 g acetoin/l from 50 g xylose/l, with a yield of 0.46 g/g xylose. Both the titer and yield were 39% higher than those of control strain BSK19A4. As a chassis cell, genome-reduced B. subtilis showed significantly improved capacity for the production of the overflow product acetoin from xylose compared with wild-type strain. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 24% |
Student > Master | 5 | 24% |
Student > Doctoral Student | 3 | 14% |
Researcher | 2 | 10% |
Student > Bachelor | 1 | 5% |
Other | 1 | 5% |
Unknown | 4 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 43% |
Agricultural and Biological Sciences | 3 | 14% |
Immunology and Microbiology | 1 | 5% |
Psychology | 1 | 5% |
Physics and Astronomy | 1 | 5% |
Other | 1 | 5% |
Unknown | 5 | 24% |