Title |
Chemical Profiling (HPLC-NMR & HPLC-MS), Isolation, and Identification of Bioactive Meroditerpenoids from the Southern Australian Marine Brown Alga Sargassum paradoxum
|
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Published in |
Marine Drugs, December 2014
|
DOI | 10.3390/md13010102 |
Pubmed ID | |
Authors |
Robert Brkljača, Sylvia Urban |
Abstract |
A phytochemical investigation of a southern Australian marine brown alga, Sargassum paradoxum, resulted in the isolation and identification of four new (5, 9, 10, and 15) and nine previously reported (1, 2, 6-8, and 11-14) bioactive meroditerpenoids. HPLC-NMR and HPLC-MS were central to the identification of a new unstable compound, sargahydroquinal (9), and pivotal in the deconvolution of eight (1, 2, 5-7, and 10-12) other meroditerpenoids. In particular, the complete characterization and identification of the two main constituents (1 and 2) in the crude dichloromethane extract was achieved using stop-flow HPLC-NMR and HPLC-MS. This study resulted in the first acquisition of gHMBCAD NMR spectra in the stop-flow HPLC-NMR mode for a system solely equipped with a 60 μL HPLC-NMR flow cell without the use of a cold probe, microcoil, or any pre-concentration. |
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Germany | 1 | 20% |
Unknown | 3 | 60% |
Demographic breakdown
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Scientists | 1 | 20% |
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Geographical breakdown
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Student > Ph. D. Student | 6 | 15% |
Student > Master | 4 | 10% |
Researcher | 4 | 10% |
Lecturer | 2 | 5% |
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Immunology and Microbiology | 2 | 5% |
Other | 6 | 15% |
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