Chapter title |
Visualization of lncRNA by Single-Molecule Fluorescence In Situ Hybridization
|
---|---|
Chapter number | 1 |
Book title |
Nuclear Bodies and Noncoding RNAs
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2253-6_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2252-9, 978-1-4939-2253-6
|
Authors |
Margaret Dunagin, Moran N. Cabili, John Rinn, Arjun Raj, Dunagin, Margaret, Cabili, Moran N., Rinn, John, Raj, Arjun |
Abstract |
Single-molecule RNA fluorescence in situ hybridization is a technique that holds great potential for the study of long noncoding RNA. It enables quantification and spatial resolution of single RNA molecules within cells via hybridization of multiple, labeled nucleic acid probes to a target RNA. It has recently become apparent that single-molecule RNA FISH probes targeting noncoding RNA are more prone to off-target binding yielding spurious results than when targeting mRNA. Here we present a protocol for the application of single-molecule RNA FISH to the study of noncoding RNA as well as an experimental procedure for validating legitimate signals. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 74 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 21 | 28% |
Student > Ph. D. Student | 20 | 27% |
Student > Master | 7 | 9% |
Student > Bachelor | 5 | 7% |
Student > Doctoral Student | 3 | 4% |
Other | 9 | 12% |
Unknown | 9 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 27 | 36% |
Biochemistry, Genetics and Molecular Biology | 26 | 35% |
Medicine and Dentistry | 4 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
Neuroscience | 2 | 3% |
Other | 2 | 3% |
Unknown | 11 | 15% |