| Title |
Control of morphology and virulence by ADP-ribosylation factors (Arf) in Mucor circinelloides
|
|---|---|
| Published in |
Current Genetics, December 2017
|
| DOI | 10.1007/s00294-017-0798-0 |
| Pubmed ID | |
| Authors |
J. Alberto Patiño-Medina, Guadalupe Maldonado-Herrera, Carlos Pérez-Arques, Viridiana Alejandre-Castañeda, Nancy Y. Reyes-Mares, Marco I. Valle-Maldonado, Jesus Campos-García, Rafael Ortiz-Alvarado, Irvin E. Jácome-Galarza, Martha I. Ramírez-Díaz, Victoriano Garre, Victor Meza-Carmen |
| Abstract |
Mucor circinelloides is a dimorphic fungus used to study cell differentiation that has emerged as a model to characterize mucormycosis. In this work, we identified four ADP-ribosylation factor (Arf)-encoding genes (arf1-arf4) and study their role in the morphogenesis and virulence. Arfs are key regulators of the vesicular trafficking process and are associated with both growth and virulence in fungi. Arf1 and Arf2 share 96% identity and Arf3 and Arf4 share 89% identity, which suggests that the genes arose through gene-duplication events in M. circinelloides. Transcription analysis revealed that certain arf genes are affected by dimorphism of M. circinelloides, such as the arf2 transcript, which was accumulated during yeast development. Therefore, we created knockout mutants of four arf genes to evaluate their function in dimorphism and virulence. We found that both arf1 and arf2 are required for sporulation, but these genes also perform distinct functions; arf2 participates in yeast development, whereas arf1 is involved in aerobic growth. Conversely, arf3 and arf4 play only minor roles during aerobic growth. Moreover, we observed that all single arf-mutant strains are more virulent than the wild-type strain in mouse and nematode models, with the arf3 mutant being most virulent. Lastly, arf1/arf2 and arf3/arf4 double mutations produced heterokaryon strains that did not reach the homokaryotic state, indicating that these genes participate in essential and redundant functions. Overall, this work reveals that Arfs proteins regulate important cellular processes in M. circinelloides such as morphogenesis and virulence, laying the foundation to characterize the molecular networks underlying this regulation. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 46 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 15% |
| Researcher | 6 | 13% |
| Other | 4 | 9% |
| Student > Doctoral Student | 4 | 9% |
| Student > Bachelor | 2 | 4% |
| Other | 8 | 17% |
| Unknown | 15 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 37% |
| Agricultural and Biological Sciences | 5 | 11% |
| Immunology and Microbiology | 4 | 9% |
| Environmental Science | 1 | 2% |
| Nursing and Health Professions | 1 | 2% |
| Other | 3 | 7% |
| Unknown | 15 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2017.
All research outputs
#20,456,235
of 23,012,811 outputs
Outputs from Current Genetics
#1,057
of 1,203 outputs
Outputs of similar age
#376,305
of 440,645 outputs
Outputs of similar age from Current Genetics
#22
of 25 outputs
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