Title |
Diagnostic performance of expression of PCA3, Hepsin and miR biomarkers inejaculate in combination with serum PSA for the detection of prostate cancer
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Published in |
The Prostate, January 2015
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DOI | 10.1002/pros.22942 |
Pubmed ID | |
Authors |
Matthew J. Roberts, Clement W. K. Chow, Horst Joachim Schirra, Renee Richards, Marion Buck, Luke A. Selth, Suhail A. R. Doi, Hema Samaratunga, Joanna Perry‐Keene, Diane Payton, John Yaxley, Martin F. Lavin, Robert A. Gardiner |
Abstract |
Here, we report on the evaluation of the diagnostic performance of ejaculate-derived PCA3, Hepsin, and miRNAs to complement serum PSA to detect prostate cancer. cDNA was prepared from 152 candidate specimens following RNA isolation and amplification for PSA, PCA3 and Hepsin qPCR, with 66 having adequate RNA for all three assays. Small RNA sequencing and examination of PCa-associated miRNAs miR-200b, miR-200c, miR-375 and miR-125b was performed on 20 specimens. We compared findings from prostate biopsies using D'Amico and PRIAS classifications and in relation to whole gland histopathology following radical prostatectomy. Multivariate logistic regression modeling and clinical risk (incorporating standard clinicopathological variables) were performed for all ejaculate-based markers. |
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Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 60 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 14 | 23% |
Student > Master | 9 | 15% |
Student > Ph. D. Student | 8 | 13% |
Researcher | 5 | 8% |
Student > Doctoral Student | 3 | 5% |
Other | 8 | 13% |
Unknown | 13 | 22% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 16 | 27% |
Biochemistry, Genetics and Molecular Biology | 10 | 17% |
Agricultural and Biological Sciences | 10 | 17% |
Engineering | 2 | 3% |
Chemistry | 2 | 3% |
Other | 4 | 7% |
Unknown | 16 | 27% |