| Title |
Intragenomic distribution of RTE retroelements suggests intrachromosomal movement
|
|---|---|
| Published in |
Chromosome Research, January 2015
|
| DOI | 10.1007/s10577-014-9461-5 |
| Pubmed ID | |
| Authors |
Eugenia E. Montiel, Francisco J. Ruiz-Ruano, Josefa Cabrero, Juan Alberto Marchal, Antonio Sánchez, Francisco Perfectti, María Dolores López-León, Juan Pedro M. Camacho |
| Abstract |
Much is known about the abundance of transposable elements (TEs) in eukaryotic genomes, but much is still unknown on their behaviour within cells. We employ here a combination of cytological, molecular and genomic approaches providing information on the intragenomic distribution and behaviour of non-long terminal repeat (LTR) retrotransposon-like elements (RTE). We microdissected every chromosome in a single first meiotic metaphase cell of the grasshopper Eyprepocnemis plorans and polymerase chain reaction (PCR) amplified a fragment of the RTE reverse transcriptase gene with specific primers. PCR products were cloned and 139 clones were sequenced. Analysis of molecular variance (AMOVA) showed significant intragenomic structure for these elements, with 4.6 % of molecular variance being found between chromosomes. A maximum likelihood tree built with the RTE sequences revealed the frequent presence of two or more elements showing very high similarity and being located on the same chromosome, thus suggesting intrachromosome movement. The 454 pyrosequencing of genomic DNA gave strong support to the microdissection results and provided evidence for the existence of 5' truncated elements. Our results thus indicate a tendency of RTE elements to reinsert into the same chromosome from where they were transcribed, which could be achieved if retrotranscription and insertion takes place immediately after transcription. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 75% |
| Members of the public | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 2 | 7% |
| United Kingdom | 1 | 3% |
| Canada | 1 | 3% |
| Spain | 1 | 3% |
| United States | 1 | 3% |
| Unknown | 24 | 80% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 30% |
| Student > Doctoral Student | 6 | 20% |
| Student > Ph. D. Student | 5 | 17% |
| Student > Master | 4 | 13% |
| Student > Bachelor | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 2 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 53% |
| Biochemistry, Genetics and Molecular Biology | 10 | 33% |
| Environmental Science | 1 | 3% |
| Immunology and Microbiology | 1 | 3% |
| Unknown | 2 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 July 2022.
All research outputs
#13,960,695
of 22,835,198 outputs
Outputs from Chromosome Research
#315
of 508 outputs
Outputs of similar age
#181,659
of 351,932 outputs
Outputs of similar age from Chromosome Research
#6
of 15 outputs
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