| Title |
Structural and functional analysis of a novel psychrophilic β-mannanase from Glaciozyma antarctica PI12
|
|---|---|
| Published in |
Perspectives in Drug Discovery and Design, May 2014
|
| DOI | 10.1007/s10822-014-9751-1 |
| Pubmed ID | |
| Authors |
Sepideh Parvizpour, Jafar Razmara, Aizi Nor Mazila Ramli, Rosli Md Illias, Mohd Shahir Shamsir |
| Abstract |
The structure of a novel psychrophilic β-mannanase enzyme from Glaciozyma antarctica PI12 yeast has been modelled and analysed in detail. To our knowledge, this is the first attempt to model a psychrophilic β-mannanase from yeast. To this end, a 3D structure of the enzyme was first predicted using a threading method because of the low sequence identity (<30%) using MODELLER9v12 and simulated using GROMACS at varying low temperatures for structure refinement. Comparisons with mesophilic and thermophilic mannanases revealed that the psychrophilic mannanase contains longer loops and shorter helices, increases in the number of aromatic and hydrophobic residues, reductions in the number of hydrogen bonds and salt bridges and numerous amino acid substitutions on the surface that increased the flexibility and its efficiency for catalytic reactions at low temperatures. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Norway | 1 | 2% |
| Unknown | 47 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 21% |
| Student > Ph. D. Student | 8 | 17% |
| Student > Master | 7 | 15% |
| Lecturer | 3 | 6% |
| Researcher | 3 | 6% |
| Other | 6 | 13% |
| Unknown | 11 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 20 | 42% |
| Agricultural and Biological Sciences | 10 | 21% |
| Chemistry | 4 | 8% |
| Medicine and Dentistry | 1 | 2% |
| Unknown | 13 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 January 2015.
All research outputs
#22,830,981
of 25,457,858 outputs
Outputs from Perspectives in Drug Discovery and Design
#868
of 949 outputs
Outputs of similar age
#207,420
of 240,110 outputs
Outputs of similar age from Perspectives in Drug Discovery and Design
#9
of 11 outputs
Altmetric has tracked 25,457,858 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 949 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 240,110 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.