Title |
Next generation sequencing for characterizing biodiversity: promises and challenges
|
---|---|
Published in |
Genetica, January 2015
|
DOI | 10.1007/s10709-015-9816-7 |
Pubmed ID | |
Authors |
François Pompanon, Sarah Samadi |
Abstract |
DNA barcoding approaches are used to describe biodiversity by analysing specimens or environmental samples in taxonomic, phylogenetic and ecological studies. While sharing data among these disciplines would be highly valuable, this remains difficult because of contradictory requirements. The properties making a DNA barcode efficient for specimen identification or species delimitation are hardly reconcilable with those required for a powerful analysis of degraded DNA from environmental samples. The use of next generation sequencing methods open up the way towards the development of new markers (e.g., multilocus barcodes) that would overcome such limitations. However, several challenges should be taken up for coordinating actions at the interface between taxonomy, ecology, molecular biology and bioinformatics in order to develop methods and protocols compatible with both taxonomic and ecological studies. |
X Demographics
Geographical breakdown
Country | Count | As % |
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India | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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France | 1 | <1% |
Italy | 1 | <1% |
Belgium | 1 | <1% |
Unknown | 156 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 42 | 26% |
Student > Ph. D. Student | 37 | 23% |
Student > Master | 21 | 13% |
Student > Doctoral Student | 9 | 6% |
Other | 9 | 6% |
Other | 22 | 14% |
Unknown | 19 | 12% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 92 | 58% |
Environmental Science | 17 | 11% |
Biochemistry, Genetics and Molecular Biology | 11 | 7% |
Immunology and Microbiology | 3 | 2% |
Earth and Planetary Sciences | 3 | 2% |
Other | 10 | 6% |
Unknown | 23 | 14% |