| Title |
Metabolism of a sea lamprey pesticide by fish liver enzymes part A: identification and synthesis of TFM metabolites
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, December 2017
|
| DOI | 10.1007/s00216-017-0830-8 |
| Pubmed ID | |
| Authors |
Ugo Bussy, Yu-Wen Chung-Davidson, Tyler Buchinger, Ke Li, Scott A. Smith, A. Daniel Jones, Weiming Li |
| Abstract |
The sea lamprey (Petromyzon marinus) is a destructive invasive species in the Great Lakes that contributed to the collapse of native fish populations in the mid-1900s. 3-Trifluoromethyl-4-nitrophenol (TFM) is a selective pesticide that has been applied to sea lamprey infested tributaries of the Great Lakes to kill larvae since the 1960s and has reduced the populations by as much as 90%. However, the metabolism of TFM by sea lamprey and non-target species is not fully illuminated. Elucidation of TFM metabolism is critical for understanding its mode of action and possible environmental impact. Here, we describe the screening, identification, synthesis and structural characterization of TFM metabolites in livers from sea lamprey and three non-target species that differ in their ability to survive TFM exposure. We identified glucuronidation, sulfation, N-acetylation, glutathione conjugation, and aromatic nitro group reduction as potential detoxification mechanisms. Seven metabolites were synthesized for use as markers of TFM metabolism in fish. Quantitative 1H NMR was used to assay synthesized metabolite stock solutions that were then used as standard material to develop a quantitative LC-MS/MS method for TFM metabolites. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 25% |
| Student > Ph. D. Student | 4 | 14% |
| Student > Bachelor | 4 | 14% |
| Researcher | 3 | 11% |
| Unspecified | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 9 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 21% |
| Biochemistry, Genetics and Molecular Biology | 6 | 21% |
| Chemistry | 3 | 11% |
| Medicine and Dentistry | 2 | 7% |
| Unspecified | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 10 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2018.
All research outputs
#20,663,600
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Outputs from Analytical & Bioanalytical Chemistry
#6,602
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Outputs of similar age
#342,778
of 448,935 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#104
of 174 outputs
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