Title |
LC-MS-Based Metabolomics Study of Marine Bacterial Secondary Metabolite and Antibiotic Production in Salinispora arenicola
|
---|---|
Published in |
Marine Drugs, January 2015
|
DOI | 10.3390/md13010249 |
Pubmed ID | |
Authors |
Utpal Bose, Amitha K. Hewavitharana, Yi Kai Ng, Paul Nicholas Shaw, John A. Fuerst, Mark P. Hodson |
Abstract |
An LC-MS-based metabolomics approach was used to characterise the variation in secondary metabolite production due to changes in the salt content of the growth media as well as across different growth periods (incubation times). We used metabolomics as a tool to investigate the production of rifamycins (antibiotics) and other secondary metabolites in the obligate marine actinobacterial species Salinispora arenicola, isolated from Great Barrier Reef (GBR) sponges, at two defined salt concentrations and over three different incubation periods. The results indicated that a 14 day incubation period is optimal for the maximum production of rifamycin B, whereas rifamycin S and W achieve their maximum concentration at 29 days. A "chemical profile" link between the days of incubation and the salt concentration of the growth medium was shown to exist and reliably represents a critical point for selection of growth medium and harvest time. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Denmark | 1 | <1% |
South Africa | 1 | <1% |
Unknown | 144 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 34 | 23% |
Student > Master | 28 | 19% |
Researcher | 18 | 12% |
Student > Doctoral Student | 11 | 8% |
Student > Bachelor | 7 | 5% |
Other | 20 | 14% |
Unknown | 28 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 37 | 25% |
Chemistry | 30 | 21% |
Biochemistry, Genetics and Molecular Biology | 24 | 16% |
Pharmacology, Toxicology and Pharmaceutical Science | 8 | 5% |
Environmental Science | 3 | 2% |
Other | 7 | 5% |
Unknown | 37 | 25% |