| Title |
Designation of fingerprint glycopeptides for targeted glycoproteomic analysis of serum haptoglobin: insights into gastric cancer biomarker discovery
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, December 2017
|
| DOI | 10.1007/s00216-017-0811-y |
| Pubmed ID | |
| Authors |
Jua Lee, Serenus Hua, Sung Hyeon Lee, Myung Jin Oh, Jaekyung Yun, Jin Young Kim, Jae-Han Kim, Jung Hoe Kim, Hyun Joo An |
| Abstract |
Gastric cancer (GC) is one of the leading causes of cancer-related death worldwide, largely because of difficulties in early diagnosis. Despite accumulating evidence indicating that aberrant glycosylation is associated with GC, site-specific localization of the glycosylation to increase specificity and sensitivity for clinical use is still an analytical challenge. Here, we created an analytical platform with a targeted glycoproteomic approach for GC biomarker discovery. Unlike the conventional glycomic approach with untargeted mass spectrometric profiling of released glycan, our platform is characterized by three key features: it is a target-protein-specific, glycosylation-site-specific, and structure-specific platform with a one-shot enzyme reaction. Serum haptoglobin enriched by immunoaffinity chromatography was subjected to multispecific proteolysis to generate site-specific glycopeptides and to investigate the macroheterogeneity and microheterogeneity. Glycopeptides were identified and quantified by nano liquid chromatography-mass spectrometry and nano liquid chromatography-tandem mass spectrometry. Ninety-six glycopeptides, each corresponding to a unique glycan/glycosite pairing, were tracked across all cancer and control samples. Differences in abundance between the two groups were marked by particularly high magnitudes. Three glycopeptides exhibited exceptionally high control-to-cancer fold changes along with receiver operating characteristic curve areas of 1.0, indicating perfect discrimination between the two groups. From the results taken together, our platform, which provides biological information as well as high sensitivity and reproducibility, may be useful for GC biomarker discovery. Graphical abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 20% |
| Netherlands | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 3 | 60% |
| Science communicators (journalists, bloggers, editors) | 1 | 20% |
| Members of the public | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 17% |
| Student > Ph. D. Student | 5 | 14% |
| Researcher | 4 | 11% |
| Student > Postgraduate | 3 | 8% |
| Professor > Associate Professor | 3 | 8% |
| Other | 8 | 22% |
| Unknown | 7 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 25% |
| Chemistry | 6 | 17% |
| Chemical Engineering | 3 | 8% |
| Agricultural and Biological Sciences | 3 | 8% |
| Medicine and Dentistry | 2 | 6% |
| Other | 6 | 17% |
| Unknown | 7 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 May 2018.
All research outputs
#15,745,807
of 25,382,440 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,753
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Outputs of similar age
#247,258
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#60
of 182 outputs
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