Title |
First genomic insights into members of a candidate bacterial phylum responsible for wastewater bulking
|
---|---|
Published in |
PeerJ, January 2015
|
DOI | 10.7717/peerj.740 |
Pubmed ID | |
Authors |
Yuji Sekiguchi, Akiko Ohashi, Donovan H. Parks, Toshihiro Yamauchi, Gene W. Tyson, Philip Hugenholtz |
Abstract |
Filamentous cells belonging to the candidate bacterial phylum KSB3 were previously identified as the causative agent of fatal filament overgrowth (bulking) in a high-rate industrial anaerobic wastewater treatment bioreactor. Here, we obtained near complete genomes from two KSB3 populations in the bioreactor, including the dominant bulking filament, using differential coverage binning of metagenomic data. Fluorescence in situ hybridization with 16S rRNA-targeted probes specific for the two populations confirmed that both are filamentous organisms. Genome-based metabolic reconstruction and microscopic observation of the KSB3 filaments in the presence of sugar gradients indicate that both filament types are Gram-negative, strictly anaerobic fermenters capable of non-flagellar based gliding motility, and have a strikingly large number of sensory and response regulator genes. We propose that the KSB3 filaments are highly sensitive to their surroundings and that cellular processes, including those causing bulking, are controlled by external stimuli. The obtained genomes lay the foundation for a more detailed understanding of environmental cues used by KSB3 filaments, which may lead to more robust treatment options to prevent bulking. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | 30% |
Denmark | 2 | 10% |
Austria | 1 | 5% |
Netherlands | 1 | 5% |
Australia | 1 | 5% |
Chile | 1 | 5% |
Spain | 1 | 5% |
Unknown | 7 | 35% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 13 | 65% |
Members of the public | 7 | 35% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 2 | 2% |
Brazil | 2 | 2% |
Germany | 1 | 1% |
United States | 1 | 1% |
Unknown | 92 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 28 | 29% |
Researcher | 13 | 13% |
Student > Master | 11 | 11% |
Student > Bachelor | 10 | 10% |
Student > Doctoral Student | 8 | 8% |
Other | 16 | 16% |
Unknown | 12 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 38 | 39% |
Biochemistry, Genetics and Molecular Biology | 14 | 14% |
Environmental Science | 11 | 11% |
Engineering | 7 | 7% |
Immunology and Microbiology | 6 | 6% |
Other | 10 | 10% |
Unknown | 12 | 12% |