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Chromosome and Genomic Engineering in Plants

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Cover of 'Chromosome and Genomic Engineering in Plants'

Table of Contents

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    Book Overview
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    Chapter 1 Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences
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    Chapter 2 Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase
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    Chapter 3 Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase-Mediated Gene Stacking
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    Chapter 4 Chromosome and Genomic Engineering in Plants
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    Chapter 5 One-Step Generation of Chromosomal Rearrangements in Rice
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    Chapter 6 Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana
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    Chapter 7 Chromosome and Genomic Engineering in Plants
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    Chapter 8 CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases
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    Chapter 9 Chromosome and Genomic Engineering in Plants
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    Chapter 10 Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination
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    Chapter 11 Chromosome and Genomic Engineering in Plants
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    Chapter 12 Chromosome and Genomic Engineering in Plants
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    Chapter 13 Image Analysis of DNA Fiber and Nucleus in Plants
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    Chapter 14 Chromosome and Genomic Engineering in Plants
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    Chapter 15 Chromosome and Genomic Engineering in Plants
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    Chapter 16 Chromosome and Genomic Engineering in Plants
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    Chapter 17 Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements
Attention for Chapter 10: Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination
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Chapter title
Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination
Chapter number 10
Book title
Chromosome and Genomic Engineering in Plants
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-4931-1_10
Pubmed ID
Book ISBNs
978-1-4939-4929-8, 978-1-4939-4931-1
Authors

Ayako Nishizawa-Yokoi, Hiroaki Saika, Seiichi Toki

Abstract

Positive-negative selection using hygromycin phosphotransferase (hpt) and diphtheria toxin A-fragment (DT-A) as positive and negative selection markers, respectively, allows enrichment of cells harboring target genes modified via gene targeting (GT). We have developed a successful GT system employing positive-negative selection and subsequent precise marker excision via the piggyBac transposon derived from the cabbage looper moth to introduce desired modifications into target genes in the rice genome. This approach could be applied to the precision genome editing of almost all endogenous genes throughout the genome, at least in rice.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 33%
Unknown 2 67%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 33%
Unknown 2 67%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2017.
All research outputs
#20,458,307
of 23,015,156 outputs
Outputs from Methods in molecular biology
#9,940
of 13,156 outputs
Outputs of similar age
#331,794
of 394,707 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
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So far Altmetric has tracked 13,156 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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