| Title |
Molecular analysis of the low-temperature Escherichia coli phage vB_EcoS_NBD2
|
|---|---|
| Published in |
Archives of Virology, October 2017
|
| DOI | 10.1007/s00705-017-3589-5 |
| Pubmed ID | |
| Authors |
Laura Kaliniene, Lidija Truncaitė, Eugenijus Šimoliūnas, Aurelija Zajančkauskaitė, Monika Vilkaitytė, Algirdas Kaupinis, Martynas Skapas, Rolandas Meškys |
| Abstract |
A novel low-temperature Escherichia coli phage vB_EcoS_NBD2 was isolated in Lithuania from agricultural soil. With an optimum temperature for plating around 20 °C, vB_EcoS_NBD2 efficiently produced plaques on Escherichia coli NovaBlue (DE3) at a temperature range of 10-30 °C, yet failed to plate at temperatures above 35 °C. Phage vB_EcoS_NBD2 virions have a siphoviral morphology with an isometric head (65 nm in diameter), and a non-contractile flexible tail (170 nm). The 51,802-bp genome of vB_EcoS_NBD2 has a G + C content of 49.8%, and contains 87 probable protein-encoding genes as well as 1 gene for tRNA(Ser). Comparative sequence analysis revealed that 22 vB_EcoS_NBD2 ORFs encode unique proteins that have no reliable identity to database entries. Based on homology to biologically defined proteins and/or proteomics analysis, 36 vB_EcoS_NBD2 ORFs were given a putative functional annotation, including 20 genes coding for morphogenesis-related proteins and 13 associated with DNA metabolism. Phylogenetic analysis revealed that vB_EcoS_NBD2 belongs to the subfamily Tunavirinae, but cannot be assigned to any genus currently recognized by ICTV. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 17% |
| Student > Bachelor | 4 | 17% |
| Student > Ph. D. Student | 3 | 13% |
| Student > Master | 3 | 13% |
| Lecturer > Senior Lecturer | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 8 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 25% |
| Agricultural and Biological Sciences | 2 | 8% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Environmental Science | 1 | 4% |
| Immunology and Microbiology | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 11 | 46% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 January 2018.
All research outputs
#18,581,651
of 23,015,156 outputs
Outputs from Archives of Virology
#3,057
of 4,208 outputs
Outputs of similar age
#248,412
of 324,363 outputs
Outputs of similar age from Archives of Virology
#29
of 69 outputs
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