Chapter title |
Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria
|
---|---|
Chapter number | 21 |
Book title |
Host-Pathogen Interactions
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7604-1_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7603-4, 978-1-4939-7604-1
|
Authors |
Jochen Schmid, Broder Rühmann, Volker Sieber, Lorena Romero-Jiménez, Juan Sanjuán, Daniel Pérez-Mendoza |
Abstract |
Bacterial exopolysaccharides (EPS) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors. They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion and biofilm formation. Biosynthesis of a growing number of EPS has been reported to be regulated by the ubiquitous second messenger c-di-GMP, which promotes the transition to a biofilm mode of growth in an intimate association with the eukaryotic host. Here we describe a strategy based on the combination of an approach to artificially increase the intracellular level of c-di-GMP in virtually any gram-negative bacteria with a high throughput screening (HTS) for the identification of monosaccharide composition and carbohydrate fingerprinting of novel EPS, or modified variants, that can be involved in host-bacteria interactions. |
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United States | 2 | 40% |
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Unknown | 2 | 40% |
Demographic breakdown
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Scientists | 3 | 60% |
Members of the public | 2 | 40% |
Mendeley readers
Geographical breakdown
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Unknown | 14 | 100% |
Demographic breakdown
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Unspecified | 2 | 14% |
Student > Bachelor | 1 | 7% |
Student > Master | 1 | 7% |
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Unknown | 5 | 36% |