| Title |
Biogenic synthesis of silver nanoparticles using Piper betle aqueous extract and evaluation of its anti-quorum sensing and antibiofilm potential against uropathogens with cytotoxic effects: an in vitro and in vivo approach
|
|---|---|
| Published in |
Environmental Science and Pollution Research, December 2017
|
| DOI | 10.1007/s11356-017-1049-0 |
| Pubmed ID | |
| Authors |
Ramanathan Srinivasan, Loganathan Vigneshwari, Tamilselvam Rajavel, Ravindran Durgadevi, Arunachalam Kannappan, Krishnaswamy Balamurugan, Kasi Pandima Devi, Arumugam Veera Ravi |
| Abstract |
Urinary tract infections are the utmost common bacterial infections caused by Proteus mirabilis, Pseudomonas aeruginosa, Escherichia coli, and Serratia marcescens. These uropathogens resist the action of several antibiotics due to their ability to form biofilms. Most of these bacterial pathogens use the quorum sensing (QS) machinery to co-ordinate their cells and regulate several virulence factors and biofilm formation. On the other hand, the anti-quorum sensing (anti-QS) and antibiofilm potential of silver nanoparticles have been well reported against certain bacterial pathogens, but to the best of our knowledge, no report is available against the pathogenicity of uropathogens in particular S. marcescens and P. mirabilis. Therefore, the present study is primarily focused on the anti-QS and antibiofilm potential of Piper betle-based synthesized silver nanoparticles (PbAgNPs) against S. marcescens and P. mirabilis. Initially, the silver nanoparticles were synthesized by the aqueous extract of P. betle and characterized by UV-absorbance spectroscopy, XRD, FT-IR, SEM, TEM, and DLS. The synthesized silver nanoparticles were assessed for their anti-QS activity and the obtained results revealed that the PbAgNPs inhibited the QS-mediated virulence factors such as prodigiosin, protease, biofilm formation, exopolysaccharides and hydrophobicity productions in uropathogens. The gene expression analysis divulged the downregulation of fimA, fimC, flhD, and bsmB genes in S. marcescens and flhB, flhD, and rsbA genes in P. mirabilis, respectively. The in vivo Caenorhabditis elegans assays revealed the non-toxic and anti-adherence efficiency of PbAgNPs. Furthermore, the non-toxic effect of PbAgNPs was also confirmed through peripheral blood mononuclear cells and normal lung epithelial cells. Therefore, the contemporary study demonstrates the use of PbAgNPs as a possible alternative toward conventional antibiotics in controlling QS and biofilm-related uropathogen infections. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 83% |
| Scientists | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 104 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 104 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 13% |
| Student > Ph. D. Student | 11 | 11% |
| Student > Bachelor | 10 | 10% |
| Student > Master | 9 | 9% |
| Student > Doctoral Student | 5 | 5% |
| Other | 8 | 8% |
| Unknown | 47 | 45% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 11% |
| Agricultural and Biological Sciences | 8 | 8% |
| Immunology and Microbiology | 7 | 7% |
| Chemistry | 5 | 5% |
| Medicine and Dentistry | 4 | 4% |
| Other | 20 | 19% |
| Unknown | 49 | 47% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 January 2018.
All research outputs
#7,885,617
of 23,911,072 outputs
Outputs from Environmental Science and Pollution Research
#1,730
of 9,883 outputs
Outputs of similar age
#154,846
of 448,337 outputs
Outputs of similar age from Environmental Science and Pollution Research
#53
of 232 outputs
Altmetric has tracked 23,911,072 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 9,883 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done well, scoring higher than 82% of its peers.
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We're also able to compare this research output to 232 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.