Title |
Combined therapy with CTL cells and oncolytic adenovirus expressing IL-15-induced enhanced antitumor activity
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Published in |
Tumor Biology, January 2015
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DOI | 10.1007/s13277-015-3098-7 |
Pubmed ID | |
Authors |
Yang Yan, Songyan Li, Tingting Jia, Xiaohui Du, Yingxin Xu, Yunshan Zhao, Li Li, Kai Liang, Wentao Liang, Huiwei Sun, Rong Li |
Abstract |
Addition of immunoregulation factor to an oncolytic adenovirus being constructed is a developmental step in tumor gene therapy; however, cytokine IL-15 has not been frequently used as a potential cancer therapy agent. Here, we constructed an E2F-1 promoter oncolytic adenovirus based on type 5 adenovirus, which induces viral replication and proliferation in targeted tumor cells. We inserted the IL-15 gene into the E3 region of the model and found that human IL-15 expressing oncolytic adenovirus (Ad-E2F/IL15) shows a more intense antitumor effect than simple oncolytic viruses (Ad-E2F) do. Precisely because IL-15 can activate natural killer (NK) cells, CD8(+)T cells, and other immune cells, in antitumor therapy, Ad-E2F/IL15 was used in combination with cytotoxic T lymphocytes (CTL) to create a virus that can induce IL-15 gene expression while lysing tumors and stimulating the activity and function of adoptive immune cells. The therapeutic effect of this therapy is clearly stronger than that of a single application of oncolytic viruses or CTL, and hence, it could be a potential new tumor therapy. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 4% |
Unknown | 22 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 5 | 22% |
Researcher | 4 | 17% |
Student > Master | 4 | 17% |
Student > Ph. D. Student | 2 | 9% |
Professor | 1 | 4% |
Other | 3 | 13% |
Unknown | 4 | 17% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 22% |
Agricultural and Biological Sciences | 4 | 17% |
Medicine and Dentistry | 4 | 17% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Veterinary Science and Veterinary Medicine | 1 | 4% |
Other | 2 | 9% |
Unknown | 6 | 26% |