| Title |
Evidence of G-protein-coupled receptor and substrate transporter heteromerization at a single molecule level
|
|---|---|
| Published in |
Cellular and Molecular Life Sciences, December 2017
|
| DOI | 10.1007/s00018-017-2728-1 |
| Pubmed ID | |
| Authors |
Jana Fischer, Gunnar Kleinau, Claudia Rutz, Denise Zwanziger, Noushafarin Khajavi, Anne Müller, Maren Rehders, Klaudia Brix, Catherine L. Worth, Dagmar Führer, Heiko Krude, Burkhard Wiesner, Ralf Schülein, Heike Biebermann |
| Abstract |
G-protein-coupled receptors (GPCRs) can constitute complexes with non-GPCR integral membrane proteins, while such interaction has not been demonstrated at a single molecule level so far. We here investigated the potential interaction between the thyrotropin receptor (TSHR) and the monocarboxylate transporter 8 (MCT8), a member of the major facilitator superfamily (MFS), using fluorescence cross-correlation spectroscopy (FCCS). Both the proteins are expressed endogenously on the basolateral plasma membrane of the thyrocytes and are involved in stimulation of thyroid hormone production and release. Indeed, we demonstrate strong interaction between both the proteins which causes a suppressed activation of Gq/11 by TSH-stimulated TSHR. Thus, we provide not only evidence for a novel interaction between the TSHR and MCT8, but could also prove this interaction on a single molecule level. Moreover, this interaction forces biased signaling at the TSHR. These results are of general interest for both the GPCR and the MFS research fields. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 25% |
| Student > Bachelor | 4 | 14% |
| Researcher | 4 | 14% |
| Professor | 1 | 4% |
| Other | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 9 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 29% |
| Agricultural and Biological Sciences | 3 | 11% |
| Chemistry | 2 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Immunology and Microbiology | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 10 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 January 2018.
All research outputs
#16,031,680
of 23,794,258 outputs
Outputs from Cellular and Molecular Life Sciences
#3,071
of 4,151 outputs
Outputs of similar age
#274,310
of 445,735 outputs
Outputs of similar age from Cellular and Molecular Life Sciences
#32
of 49 outputs
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