| Title |
Rapid detection of mutagen induced micronucleated erythrocytes by flow cytometry
|
|---|---|
| Published in |
Histochemistry and Cell Biology, September 1982
|
| DOI | 10.1007/bf00496738 |
| Pubmed ID | |
| Authors |
K. -J. Hutter, M. Stöhr |
| Abstract |
The micronucleus test is a cytogenetic method for the screening of mutagens and carcinogens which exhibit clastogenic mechanisms of action. After application of clastogenic agents chromosomal fragments or even whole chromosomes can remain as conspicuous structures (micronuclei) in a small fraction of anucleated polychromatic erythrocytes which can be visually scored using a microscope following staining with May-Grünwald-Giemsa solutions. These time-consuming, painstaking, and tedious manual evaluations are often sources of unreliability and uncertainty. Here, a fluorescence technique is presented which applies DNA and protein fluorochromes to discriminate normal anucleated erythrocytes from micronucleated erythrocytes using a fluorescence microscope. This method is particularly tailored to be applied to flow cytometric instrumentation. Data obtained manually and automatically in flow show a strong linear correlation with high significance (r = 0.96) as far as the percentage of micronucleated erythrocytes as an indicator for the mutagenicity of a given drug is concerned. These results have been obtained by means of the established clastogens cyclophosphamide and mitomycin C. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 20% |
| Researcher | 2 | 20% |
| Other | 1 | 10% |
| Lecturer | 1 | 10% |
| Professor | 1 | 10% |
| Other | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 2 | 20% |
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Unspecified | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Other | 2 | 20% |
| Unknown | 1 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2019.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Histochemistry and Cell Biology
#306
of 1,236 outputs
Outputs of similar age
#2,023
of 7,258 outputs
Outputs of similar age from Histochemistry and Cell Biology
#1
of 1 outputs
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