| Title |
Preparation of pure cell cultures by cloning
|
|---|---|
| Published in |
Methods in Cell Science, April 2000
|
| DOI | 10.1023/a:1009838416621 |
| Pubmed ID | |
| Authors |
Douglas C. McFarland |
| Abstract |
Due to the heterogeneous nature of animal organs, primary cell cultures potentially contain more than one type of cell. Interpretation of data arising from studies using these mixed cultures can lead to difficulties, because it may not be possible to ascertain which of the cell types present may have responded to a given treatment. While a number of procedures have been used to enrich the cell population of interest, many scientists have resorted to cloning of cells in order to insure the purity of cell cultures. Three major strategies have been used to produce clones, namely, the dilution technique, cloning ring technique, and robotic cell transfer. Successful cloning is dependent on optimization of attachment substrata, basal media composition, serum source, and growth factor/hormone additions to support proliferation of the cell type at clonal (low) density. |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 2% |
| Poland | 1 | 2% |
| Unknown | 40 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 24% |
| Student > Ph. D. Student | 9 | 21% |
| Researcher | 6 | 14% |
| Professor > Associate Professor | 5 | 12% |
| Student > Master | 3 | 7% |
| Other | 6 | 14% |
| Unknown | 3 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 48% |
| Biochemistry, Genetics and Molecular Biology | 7 | 17% |
| Medicine and Dentistry | 5 | 12% |
| Immunology and Microbiology | 3 | 7% |
| Chemistry | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 3 | 7% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 April 2024.
All research outputs
#3,798,945
of 25,374,647 outputs
Outputs from Methods in Cell Science
#60
of 1,026 outputs
Outputs of similar age
#3,672
of 40,972 outputs
Outputs of similar age from Methods in Cell Science
#1
of 2 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,026 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 40,972 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them