| Title |
Cannabis Users Show Enhanced Expression of CB1-5HT2A Receptor Heteromers in Olfactory Neuroepithelium Cells
|
|---|---|
| Published in |
Molecular Neurobiology, January 2018
|
| DOI | 10.1007/s12035-017-0833-7 |
| Pubmed ID | |
| Authors |
Liliana Galindo, Estefanía Moreno, Fernando López-Armenta, Daniel Guinart, Aida Cuenca-Royo, Mercè Izquierdo-Serra, Laura Xicota, Cristina Fernandez, Esther Menoyo, José M. Fernández-Fernández, Gloria Benítez-King, Enric I. Canela, Vicent Casadó, Víctor Pérez, Rafael de la Torre, Patricia Robledo |
| Abstract |
Cannabinoid CB1 receptors (CB1R) and serotonergic 2A receptors (5HT2AR) form heteromers in the brain of mice where they mediate the cognitive deficits produced by delta-9-tetrahydrocannabinol. However, it is still unknown whether the expression of this heterodimer is modulated by chronic cannabis use in humans. In this study, we investigated the expression levels and functionality of CB1R-5HT2AR heteromers in human olfactory neuroepithelium (ON) cells of cannabis users and control subjects, and determined their molecular characteristics through adenylate cyclase and the ERK 1/2 pathway signaling studies. We also assessed whether heteromer expression levels correlated with cannabis consumption and cognitive performance in neuropsychological tests. ON cells from controls and cannabis users expressed neuronal markers such as βIII-tubulin and nestin, displayed similar expression levels of genes related to cellular self-renewal, stem cell differentiation, and generation of neural crest cells, and showed comparable Na+ currents in patch clamp recordings. Interestingly, CB1R-5HT2AR heteromer expression was significantly increased in cannabis users and positively correlated with the amount of cannabis consumed, and negatively with age of onset of cannabis use. In addition, a negative correlation was found between heteromer expression levels and attention and working memory performance in cannabis users and control subjects. Our findings suggest that cannabis consumption regulates the formation of CB1R-5HT2AR heteromers, and may have a key role in cognitive processing. These heterodimers could be potential new targets to develop treatment alternatives for cognitive impairments. |
X Demographics
The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 44% |
| United Kingdom | 3 | 33% |
| Spain | 1 | 11% |
| Unknown | 1 | 11% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 56% |
| Scientists | 4 | 44% |
Mendeley readers
The data shown below were compiled from readership statistics for 106 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 106 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 19% |
| Researcher | 15 | 14% |
| Student > Bachelor | 14 | 13% |
| Other | 9 | 8% |
| Student > Master | 7 | 7% |
| Other | 17 | 16% |
| Unknown | 24 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 15 | 14% |
| Medicine and Dentistry | 15 | 14% |
| Biochemistry, Genetics and Molecular Biology | 11 | 10% |
| Chemistry | 7 | 7% |
| Psychology | 7 | 7% |
| Other | 20 | 19% |
| Unknown | 31 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 October 2022.
All research outputs
#2,500,986
of 23,577,761 outputs
Outputs from Molecular Neurobiology
#291
of 3,562 outputs
Outputs of similar age
#58,502
of 444,943 outputs
Outputs of similar age from Molecular Neurobiology
#10
of 84 outputs
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