| Title |
Protein Disulfide Isomerase Silence Inhibits Inflammatory Functions of Macrophages by Suppressing Reactive Oxygen Species and NF-κB Pathway
|
|---|---|
| Published in |
Inflammation, January 2018
|
| DOI | 10.1007/s10753-017-0717-z |
| Pubmed ID | |
| Authors |
Yinbo Xiao, Chaohong Li, Minghui Gu, Haixing Wang, Weishen Chen, Guotian Luo, Guangpu Yang, Ziji Zhang, Yangchun Zhang, Guoyan Xian, Ziqing Li, Puyi Sheng |
| Abstract |
Macrophages play an essential role in inflammation. Protein disulfide isomerase (PDI) is central to the redox system, which is closely linked with the inflammatory function of macrophages. However, the relationship between PDI and inflammation is still unknown. In this study, we tested the effects of PDI on inflammatory responses in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). Using CRISPR/Cas9 system, we found that PDI knockout suppressed migration, M1 polarization, and secretion of tumor necrosis factor-α (TNF-α) and interluekin-6 (IL-6). The repression of these inflammatory processes was accompanied by decreased production of reactive oxygen species (ROS). PDI ablation also inactivated the phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activated the phosphorylation of NF-κB inhibitor alpha (IκBα). These findings demonstrate that PDI knockout inhibits the inflammatory function of macrophages by decreasing ROS production and inactivating NF-κB pathway. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 16% |
| Student > Doctoral Student | 3 | 12% |
| Researcher | 3 | 12% |
| Student > Bachelor | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 8 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 16% |
| Medicine and Dentistry | 4 | 16% |
| Agricultural and Biological Sciences | 2 | 8% |
| Neuroscience | 2 | 8% |
| Immunology and Microbiology | 2 | 8% |
| Other | 3 | 12% |
| Unknown | 8 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2018.
All research outputs
#20,458,307
of 23,015,156 outputs
Outputs from Inflammation
#724
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Outputs of similar age
#378,057
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Outputs of similar age from Inflammation
#12
of 17 outputs
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