| Title |
High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination (SMI) microscopy
|
|---|---|
| Published in |
Chromosome Research, May 2008
|
| DOI | 10.1007/s10577-008-1238-2 |
| Pubmed ID | |
| Authors |
Jürgen Reymann, David Baddeley, Manuel Gunkel, Paul Lemmer, Werner Stadter, Thibaud Jegou, Karsten Rippe, Christoph Cremer, Udo Birk |
| Abstract |
Spatially modulated illumination (SMI) microscopy is a method of wide field fluorescence microscopy featuring interferometric illumination, which delivers structural information about nanoscale architecture in fluorescently labelled cells. The first prototype of the SMI microscope proved its applicability to a wide range of biological questions. For the SMI live cell imaging this system was enhanced in terms of the development of a completely new upright configuration. This so called Vertico-SMI transfers the advantages of SMI nanoscaling to vital biological systems, and is shown to work consistently at different temperatures using both oil- and water-immersion objective lenses. Furthermore, we increased the speed of data acquisition to minimize errors in the detection signal resulting from cellular or object movement. By performing accurate characterization, the present Vertico-SMI now offers a fully-fledged microscope enabling a complete three-dimensional (3D) SMI data stack to be acquired in less than 2 seconds. We have performed live cell measurements of a tet-operator repeat insert in U2OS cells, which provided the first in vivo signatures of subnuclear complexes. Furthermore, we have successfully implemented an optional optical configuration allowing the generation of high-resolution localization microscopy images of a nuclear pore complex distribution. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 137 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 1% |
| France | 2 | 1% |
| Czechia | 2 | 1% |
| United States | 2 | 1% |
| United Kingdom | 1 | <1% |
| Ireland | 1 | <1% |
| Japan | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 125 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 33 | 24% |
| Student > Ph. D. Student | 29 | 21% |
| Student > Master | 17 | 12% |
| Student > Doctoral Student | 13 | 9% |
| Student > Bachelor | 10 | 7% |
| Other | 21 | 15% |
| Unknown | 14 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 42 | 31% |
| Physics and Astronomy | 27 | 20% |
| Engineering | 16 | 12% |
| Biochemistry, Genetics and Molecular Biology | 10 | 7% |
| Chemistry | 8 | 6% |
| Other | 16 | 12% |
| Unknown | 18 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 April 2024.
All research outputs
#3,429,473
of 24,387,992 outputs
Outputs from Chromosome Research
#47
of 528 outputs
Outputs of similar age
#9,784
of 81,540 outputs
Outputs of similar age from Chromosome Research
#2
of 8 outputs
Altmetric has tracked 24,387,992 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 528 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 81,540 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 6 of them.