| Title |
Bacterial lipases: an overview of production, purification and biochemical properties
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, February 2004
|
| DOI | 10.1007/s00253-004-1568-8 |
| Pubmed ID | |
| Authors |
R. Gupta, N. Gupta, P. Rathi |
| Abstract |
Lipases, triacylglycerol hydrolases, are an important group of biotechnologically relevant enzymes and they find immense applications in food, dairy, detergent and pharmaceutical industries. Lipases are by and large produced from microbes and specifically bacterial lipases play a vital role in commercial ventures. Some important lipase-producing bacterial genera include Bacillus, Pseudomonas and Burkholderia. Lipases are generally produced on lipidic carbon, such as oils, fatty acids, glycerol or tweens in the presence of an organic nitrogen source. Bacterial lipases are mostly extracellular and are produced by submerged fermentation. The enzyme is most commonly purified by hydrophobic interaction chromatography, in addition to some modern approaches such as reverse micellar and aqueous two-phase systems. Most lipases can act in a wide range of pH and temperature, though alkaline bacterial lipases are more common. Lipases are serine hydrolases and have high stability in organic solvents. Besides these, some lipases exhibit chemo-, regio- and enantioselectivity. The latest trend in lipase research is the development of novel and improved lipases through molecular approaches such as directed evolution and exploring natural communities by the metagenomic approach. |
Mendeley readers
The data shown below were compiled from readership statistics for 1,096 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 6 | <1% |
| Colombia | 5 | <1% |
| Germany | 4 | <1% |
| United States | 3 | <1% |
| Brazil | 3 | <1% |
| France | 3 | <1% |
| Portugal | 2 | <1% |
| Belgium | 2 | <1% |
| Uruguay | 1 | <1% |
| Other | 10 | <1% |
| Unknown | 1057 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 190 | 17% |
| Student > Master | 185 | 17% |
| Student > Bachelor | 173 | 16% |
| Researcher | 129 | 12% |
| Student > Doctoral Student | 64 | 6% |
| Other | 136 | 12% |
| Unknown | 219 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 362 | 33% |
| Biochemistry, Genetics and Molecular Biology | 180 | 16% |
| Chemistry | 113 | 10% |
| Engineering | 58 | 5% |
| Environmental Science | 37 | 3% |
| Other | 99 | 9% |
| Unknown | 247 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2024.
All research outputs
#5,446,994
of 25,374,647 outputs
Outputs from Applied Microbiology and Biotechnology
#1,292
of 8,290 outputs
Outputs of similar age
#16,875
of 143,369 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#13
of 35 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,290 research outputs from this source. They receive a mean Attention Score of 4.4. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 143,369 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.