| Title |
Pyramiding of transgenic Pm3 alleles in wheat results in improved powdery mildew resistance in the field
|
|---|---|
| Published in |
Theoretical and Applied Genetics, January 2018
|
| DOI | 10.1007/s00122-017-3043-9 |
| Pubmed ID | |
| Authors |
Teresa Koller, Susanne Brunner, Gerhard Herren, Severine Hurni, Beat Keller |
| Abstract |
The combined effects of enhanced total transgene expression level and allele-specificity combination in transgenic allele-pyramided Pm3 wheat lines result in improved powdery mildew field resistance without negative pleiotropic effects. Allelic Pm3 resistance genes of wheat confer race-specific resistance to powdery mildew (Blumeria graminis f. sp. tritici, Bgt) and encode nucleotide-binding domain, leucine-rich repeat (NLR) receptors. Transgenic wheat lines overexpressing alleles Pm3a, b, c, d, f, and g have previously been generated by transformation of cultivar Bobwhite and tested in field trials, revealing varying degrees of powdery mildew resistance conferred by the transgenes. Here, we tested four transgenic lines each carrying two pyramided Pm3 alleles, which were generated by crossbreeding of lines transformed with single Pm3 alleles. All four allele-pyramided lines showed strongly improved powdery mildew resistance in the field compared to their parental lines. The improved resistance results from the two effects of enhanced total transgene expression levels and allele-specificity combinations. In contrast to leaf segment tests on greenhouse-grown seedlings, no allelic suppression was observed in the field. Plant development and yield scores of the pyramided lines were similar to the mean scores of the corresponding parental lines, and thus, the allele pyramiding did not cause any negative effects. On the contrary, in pyramided line, Pm3b × Pm3f normal plant development was restored compared to the delayed development and reduced seed set of parental line Pm3f. Allele-specific RT qPCR revealed additive transgene expression levels of the two Pm3 alleles in the pyramided lines. A positive correlation between total transgene expression level and powdery mildew field resistance was observed. In summary, allele pyramiding of Pm3 transgenes proved to be successful in enhancing powdery mildew field resistance. |
X Demographics
The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 2 | 9% |
| United States | 2 | 9% |
| United Kingdom | 2 | 9% |
| Turkey | 1 | 5% |
| Guatemala | 1 | 5% |
| Switzerland | 1 | 5% |
| Thailand | 1 | 5% |
| Germany | 1 | 5% |
| Unknown | 11 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 15 | 68% |
| Scientists | 7 | 32% |
Mendeley readers
The data shown below were compiled from readership statistics for 75 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 75 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 24% |
| Researcher | 9 | 12% |
| Student > Bachelor | 7 | 9% |
| Student > Master | 5 | 7% |
| Other | 4 | 5% |
| Other | 8 | 11% |
| Unknown | 24 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 37 | 49% |
| Biochemistry, Genetics and Molecular Biology | 7 | 9% |
| Social Sciences | 2 | 3% |
| Environmental Science | 1 | 1% |
| Materials Science | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 26 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 77. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 July 2018.
All research outputs
#551,544
of 25,392,205 outputs
Outputs from Theoretical and Applied Genetics
#4
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Outputs of similar age
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Outputs of similar age from Theoretical and Applied Genetics
#1
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